Effects of transforming growth factor-beta1 on cell motility, collagen gel contraction, myofibroblastic differentiation, and extracellular matrix expression of human adipose-derived stem cell
Effects of transforming growth factor-beta1 on cell motility, collagen gel contraction, myofibroblastic differentiation, and extracellular matrix expression of human adipose-derived stem cell
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DOI：
10.1007/s13577-012-0049-0
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发表时间：
2012-01-01
期刊：
影响因子：
4.3
通讯作者：
Kusumoto, Kenji
中科院分区：
文献类型：
--
作者：
Kakudo, Natsuko;Kushida, Satoshi;Kusumoto, Kenji
人类脂肪源性干细胞（ASC）是成体多能干细胞，近年来它们在整形外科中的用途引起了人们的关注。尽管人们一直期望ASC可能在伤口修复和再生中发挥作用，但迄今为止还没有研究检查ASC在促进伤口愈合的机制中的作用。转化生长因子-β 1（TGF-β 1）是一种强有力的候选细胞因子，用于触发间充质干细胞迁移、细胞外基质的构建以及ASC分化为肌成纤维细胞。细胞增殖、运动和分化以及细胞外基质的产生在伤口愈合中起重要作用。我们已经评估了ASCs的增殖能力和分化成表型肌成纤维细胞的潜力，以及它们的细胞运动性和胶原凝胶收缩能力，当与TGF-β 1一起培养时。使用伤口愈合测定分析细胞运动性。分化为肌成纤维细胞的ASCs表达α-平滑肌肌动蛋白基因，其蛋白表达通过免疫组化检测。实时荧光定量RT-PCR检测ASCs细胞外基质的表达。基于这些结果，我们得出结论，人类ASCs具有细胞运动，细胞外基质基因表达，凝胶收缩和分化成肌成纤维细胞的潜力，因此，可能在伤口愈合过程中发挥重要作用。
Human adipose-derived stem cells (ASCs) are adult pluripotent stem cells, and their usefulness in plastic surgery has garnered attention in recent years. Although, there have been expectations that ASCs might function in wound repair and regeneration, no studies to date have examined the role of ASCs in the mechanism that promotes wound-healing. Transforming growth factor-beta1 (TGF-beta 1) is a strong candidate cytokine for the triggering of mesenchymal stem cell migration, construction of extracellular matrices, and differentiation of ASCs into myofibroblasts. Cell proliferation, motility, and differentiation, as well as extracellular matrix production, play an important role in wound-healing. We have evaluated the capacity of ASCs to proliferate and their potential to differentiate into phenotypic myofibroblasts, as well as their cell motility and collagen gel contraction ability, when cultured with TGF-beta 1. Cell motility was analyzed using a wound-healing assay. ASCs that differentiated into myofibroblasts expressed the gene for alpha-smooth muscle actin, and its protein expression was detected immunohistochemically. The extracellular matrix expression in ASCs was evaluated using real-time RT-PCR. Based on the results, we conclude that human ASCs have the potential for cell motility, extracellular matrix gene expression, gel contraction, and differentiation into myofibroblasts and, therefore, may play an important role in the wound-healing process.