Orphon spliced-leader sequences form part of a repetitive element in Angiostrongylus cantonensis.
Orphon spliced-leader sequences form part of a repetitive element in Angiostrongylus cantonensis.
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DOI：
10.1093/nar/23.6.1030
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发表时间：
1995-03
影响因子：
14.9
通讯作者：
G. Joshua;F. Perler;C. Wang
中科院分区：
文献类型：
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作者：
G. Joshua;F. Perler;C. Wang
在线虫中，22个核苷酸（nt）的剪接前导序列（SL）通常由一个多拷贝、串联重复的SL基因编码，并从SL - RNA反式剪接到一部分信使核糖核酸（mRNA）的5'端。我们发现，广州管圆线虫这种寄生线虫的多个（>100个）孤立基因座位点也编码SL。在这些位点，该序列构成一个198碱基对重复元件（命名为con - 198）的一部分。对包含con - 198序列的两个基因元件的转录情况已进行了特征分析。在一个元件（G - 2）处，转录出一种约850个核苷酸且含有内部SL的核糖核酸。在另一个元件（G - 1）处，转录发生在con - 198序列下游3kb处。
In nematodes, the 22 nucleotide (nt) spliced leader (SL) is normally encoded by a multi-copy, tandemly reiterated SL gene and is trans-spliced from SL-RNA onto the 5' end of a subset of mRNAs. We have found that the SL is also encoded at multiple (> 100) orphon genomic sites in the parasitic nematode Angiostrongylus cantonensis. At these sites the sequence forms part of a 198 bp repetitive element (designated con-198). Transcription from two genomic elements that contain the con-198 sequence has been characterised. At one element (G-2) an approximately 850 nt RNA with an internal SL is transcribed. At the other (G-1), transcription takes place 3 kb downstream of the con-198 sequence.