Immunohistochemical detection of tumor necrosis factor-alpha, other cytokines and adhesion molecules in human livers with alcoholic hepatitis
Immunohistochemical detection of tumor necrosis factor-alpha, other cytokines and adhesion molecules in human livers with alcoholic hepatitis
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酒精性肝炎人肝脏中肿瘤坏死因子-α、其他细胞因子和粘附分子的免疫组织化学检测
作者：
W. Öhlinger;H. Dinges;K. Zatloukal;S. Mair;F. Gollowitsch;H. Denk
本研究旨在探讨急性酒精性肝炎中肿瘤坏死因子α（TNFα）和白细胞介素（IL）等重要细胞因子的可能作用及其局部分布。这些细胞因子对于粘附分子如细胞间粘附分子-1（ICAM-1）和内皮白细胞粘附分子-1（ELAM-1）的表达和/或上调的众所周知的诱导能力是有待研究的另一点。此外，所提出的粘附分子的诱导也可能与酒精性肝炎特有的炎症细胞的特殊群体的激活和吸引有关。采用碱性磷酸酶抗碱性磷酸酶免疫染色技术以及单、双间接免疫荧光法对死于急性酒精性肝炎的患者的冷冻肝脏样本进行了评价。在急性酒精性肝炎中，TNFα主要在气球样肝细胞中检出，其中通常含有酒精性透明蛋白（马洛里小体）。此外，TNFα与肝细胞膜中表达的ICAM-1共分布，并出现CD 11b阳性多形核白细胞（中性粒细胞），表明β2-整联蛋白Mac-1可能作为ICAM-1的配体发挥主要作用。没有诱导ELAM-1可能被发现。在酒精性肝炎细胞因子可能是负责诱导的粘附分子ICAM-1对肝细胞膜和激活一个定义的人口的炎症细胞，从而有助于急性酒精性肝炎的特征性组织学图片与其浓度的中性粒细胞，特别是在该地区的气球马洛里体含有肝细胞。我们的研究结果与临床结果一致，即酒精性肝炎患者血清中TNFα和IL-1水平较高，并且与已经报道的肝细胞ICAM-1表达一致。
This immunohistochemical study was designed to investigate the possible contribution to and topographical distribution of some important cytokines, such as tumour necrosis factor α (TNFα) and interleukins, in acute alcoholic hepatitis. The well-known inductive capacity of these cytokines with respect to the expression and/or up-regulation of adhesion molecules, such as intercellular adhesion molecule-1 (ICAM-1) and endothelial leukocyte adhesion molecule-1 (ELAM-1), was a further point to be studied. Moreover, the proposed induction of adhesion molecules might also be associated with the activation and attraction of a special population of inflammatory cells characteristic for alcoholic hepatitis. Frozen liver samples from patients who died with signs of acute alcoholic hepatitis were evaluated using the alkaline phosphatase anti-alkaline phosphatase immunostaining technique and also single and double indirect immunofluorescence. In acute alcoholic hepatitis TNFα could be detected predominantly in ballooned hepatocytes, which often contained alcoholic hyalin (Mallory bodies). Moreover, TNFα showed a co-distribution with ICAM-1 expressed in the membranes of hepatocytes and with the occurrence of CD11b positive polymorphonuclear leukocytes (neutrophils) suggesting a possible major role of the β2-integrin Mac-1 as a ligand for ICAM-1. No induction of ELAM-1 could be found. In alcoholic hepatitis cytokines may be responsible for the induction of the adhesion molecule ICAM-1 on hepatocytic membranes and activate a defined population of inflammatory cells, thus contributing to the characteristic histological picture of acute alcoholic hepatitis with its concentration of neutrophils especially in areas with ballooned Mallory body-containing hepatocytes. Our results are in line with clinical findings showing high levels of TNFα and interleukin-1 in sera of patients with alcoholic hepatitis and with the already reported expression of ICAM-1 on hepatocytes.
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影响因子：
3.9
作者：
Pal, Nabanita;Kim, Taeyeon;Park, Jae-Seo;Cho, Eun-Bum
通讯作者：
Cho, Eun-Bum
影响因子：
4.4
作者：
Michael Loran Dustin;R. Rothlein;A. Bhan;C. Dinarello;T. Springer
通讯作者：
Michael Loran Dustin;R. Rothlein;A. Bhan;C. Dinarello;T. Springer
铁介导大鼠肝细胞代谢乙醇产生中性粒细胞趋化剂。
DOI：
10.1172/jci114999
发表时间：
1991
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Hultcrantz,R;Bissell,DM;Roll,FJ
通讯作者：
Roll,FJ
DOI：
--
发表时间：
1991-02
期刊：
The American journal of pathology
影响因子：
--
作者：
S. Chensue;D. Pauline;Terebuh;D. Remick;W. Scales;Steven L. Kunkelt
通讯作者：
S. Chensue;D. Pauline;Terebuh;D. Remick;W. Scales;Steven L. Kunkelt
暴露于乙醇的培养大鼠肝细胞对多形核白细胞产生趋化活性。
DOI：
10.1172/jci111545
发表时间：
1984
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Perez,HD;Roll,FJ;Bissell,DM;Shak,S;Goldstein,IM
通讯作者：
Goldstein,IM