CD44s and CD44v6 expression in head and neck epithelia.
CD44s and CD44v6 expression in head and neck epithelia.
复制标题
DOI：
10.1371/journal.pone.0003360
复制
发表时间：
2008
期刊：
影响因子：
3.7
通讯作者：
Gires, Olivier
中科院分区：
文献类型：
--
作者：
Mack, Brigitte;Gires, Olivier
CD44剪接变体长期以来被认为与细胞转化有关。最近，CD44的标准形式（CD44）被证明是结肠癌、乳腺癌和头颈部鳞状细胞癌（HNSCC）中癌症干细胞（CSCs）特征的一部分。这与之前关于cd44在HNSCC中表达的报道有些矛盾。本研究的目的是阐明CD44在头颈部上皮中的实际表达模式。用免疫组化方法分析CD44s和CD44v6在原发头颈部组织中的表达。所有标本的评分采用双参数系统，该系统实现了阳性细胞百分比和染色强度从-到+++ (score = %×intensity；得分300)。此外，我们还检测了淋巴细胞和HNSCC中CD44s和CD44v6的细胞表面表达。在正常上皮细胞中，CD44s和CD44v6分别在60-95%和50-80%的细胞中表达，其平均评分标准误差（SEM）分别为249.5±14.5和198±11.13。在口腔白斑和中分化癌中，CD44s和CD44v6水平略有升高（分别为278.9±7.16和242±11.7;291.8±5.88和287.3±6.88）。原位癌显示两种蛋白水平不变，而低分化癌一致表达CD44s和CD44v6水平降低。淋巴细胞和HNSCC细胞系强烈表达CD44s，但不表达CD44v6。CD44s和CD44v6的表达不能区分头颈部上皮的正常与良性或恶性。CD44s和CD44v6大量存在于头颈部组织的绝大多数细胞中，包括癌细胞。因此，CD44s作为一小部分细胞（即小于10%）代表头颈癌干细胞定义的标记物的价值可能需要修订。
CD44 splice variants are long-known as being associated with cell transformation. Recently, the standard form of CD44 (CD44s) was shown to be part of the signature of cancer stem cells (CSCs) in colon, breast, and in head and neck squamous cell carcinomas (HNSCC). This is somewhat in contradiction to previous reports on the expression of CD44s in HNSCC. The aim of the present study was to clarify the actual pattern of CD44 expression in head and neck epithelia. Expression of CD44s and CD44v6 was analysed by immunohistochemistry with specific antibodies in primary head and neck tissues. Scoring of all specimens followed a two-parameters system, which implemented percentages of positive cells and staining intensities from − to +++ (score = %×intensity; resulting max. score 300). In addition, cell surface expression of CD44s and CD44v6 was assessed in lymphocytes and HNSCC. In normal epithelia CD44s and CD44v6 were expressed in 60–95% and 50–80% of cells and yielded mean scores with a standard error of a mean (SEM) of 249.5±14.5 and 198±11.13, respectively. In oral leukoplakia and in moderately differentiated carcinomas CD44s and CD44v6 levels were slightly increased (278.9±7.16 and 242±11.7; 291.8±5.88 and 287.3±6.88). Carcinomas in situ displayed unchanged levels of both proteins whereas poorly differentiated carcinomas consistently expressed diminished CD44s and CD44v6 levels. Lymphocytes and HNSCC lines strongly expressed CD44s but not CD44v6. CD44s and CD44v6 expression does not distinguish normal from benign or malignant epithelia of the head and neck. CD44s and CD44v6 were abundantly present in the great majority of cells in head and neck tissues, including carcinomas. Hence, the value of CD44s as a marker for the definition of a small subset of cells (i.e. less than 10%) representing head and neck cancer stem cells may need revision.
登录
查看更多内容
影响因子：
3.7
作者：
Wiesner M;Zentz C;Mayr C;Wimmer R;Hammerschmidt W;Zeidler R;Moosmann A
通讯作者：
Moosmann A
DOI：
10.1083/jcb.200310024
发表时间：
2004-06-21
期刊：
The Journal of cell biology
影响因子：
--
作者：
Nagano O;Murakami D;Hartmann D;De Strooper B;Saftig P;Iwatsubo T;Nakajima M;Shinohara M;Saya H
通讯作者：
Saya H
影响因子：
4.8
作者：
Lammich, S;Okochi, M;Haass, C
通讯作者：
Haass, C
DOI：
10.1073/pnas.0703478104
发表时间：
2007-06-12
影响因子：
11.1
作者：
Dalerba, Piero;Dylla, Scott J.;Clarke, Michael F.
通讯作者：
Clarke, Michael F.
影响因子：
8.8
作者：
通讯作者：
--