Microtubule-Associated Protein 2, an Early Blood Marker of Ischemic Brain Injury
Microtubule-Associated Protein 2, an Early Blood Marker of Ischemic Brain Injury
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DOI：
10.1002/jnr.22769
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发表时间：
2012-02-01
影响因子：
4.2
通讯作者：
Kim, Yun-Bae
中科院分区：
文献类型：
--
作者：
Park, Dongsun;Joo, Seong S.;Kim, Yun-Bae
这项研究的目的是开发一种灵敏而快速的血液标记物来检测缺血性脑损伤，因为成像技术在最初的关键时间内识别损伤的能力有限，而不会造成大规模的组织破坏。大鼠大脑中动脉闭塞不同时间(0.5~3h)后再灌流。用免疫印迹法检测脑缺血和/或缺血再灌注后不同时间点脑脊液和血清中胶质纤维酸性蛋白(GFAP)和微管相关蛋白2(MAP2)的含量。脑梗塞以缺血持续时间依赖的方式观察。脑脊液中GFAP在再灌流后24小时和48小时显著升高，而血清水平无明显变化。血清MAP2水平在缺血0.5小时时显著升高，远早于闭塞后3小时观察到的轻微组织损伤。再灌注2小时后，血清MAP2水平进一步升高，即使在0.5小时和1小时的缺血大鼠中也是如此，尽管在再灌注24小时后没有观察到任何典型的组织损伤。这些结果表明，MAP2蛋白可能能够检测到早期的神经元损伤，因为这种蛋白在血液中的水平在可见的大病变出现之前就会出现峰值。因此，MAP2可能成为检测神经毒性损伤的一种新的早期标志物。(C)2011年威利期刊公司。
The aim of this study was to develop a sensitive and rapid blood marker to detect ischemic brain injury, because imaging techniques have a limited capacity to identify lesions during the first crucial hours without massive tissue destruction. Rats were subjected to middle cerebral artery occlusion for various durations (0.5-3 hr), followed by reperfusion. At different time points after ischemia and/or ischemia-reperfusion, the amounts of glial fibrillary acidic protein (GFAP) and microtubule-associated protein 2 (MAP2) in the cerebrospinal fluid (CSF) and serum were analyzed by Western blotting. Brain infarction was observed in an ischemia-duration-dependent manner. GFAP was drastically increased in the CSF 24 and 48 hr after reperfusion, without change in the serum level. Serum levels of MAP2 remarkably increased as early as 0.5 hr of ischemia, much earlier than the observation of minimal tissue injury 3 hr following occlusion. The serum MAP2 level was further increased by a short period (2 hr) of reperfusion, even in 0.5- and 1-hr ischemic rats, despite not observing any typical tissue injuries 24 hr after reperfusion. These results indicate that the MAP2 protein may be able to detect early neuronal injuries, because the level of this protein in the blood spikes before the appearance of visible macrolesions. Therefore, MAP2 could potentially be used as a novel early marker for the detection of a neurotoxic insult. (C) 2011 Wiley Periodicals, Inc.