Structure and chromosomal mapping of a highly polymorphic repetitive DNA sequence from the pseudoautosomal region of the mouse sex chromosomes.
Structure and chromosomal mapping of a highly polymorphic repetitive DNA sequence from the pseudoautosomal region of the mouse sex chromosomes.
复制标题
小鼠性染色体假常染色体区域高度多态性重复 DNA 序列的结构和染色体定位。
DOI：
10.1159/000132912
复制
发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Müller,U
中科院分区：
文献类型：
--
作者：
Harbers,K;Francke,U;Soriano,P;Jaenisch,R;Müller,U
Mov15小鼠品系的假常染色体区域由莫洛尼鼠白血病前病毒标记。侧翼的Mov15前病毒的序列进行分子克隆，并显示由31个核苷酸的串联重复序列。该重复序列的拷贝数变异最有可能解释了用来自侧翼序列的探针检测到的小鼠假常染色体区域中的多态性。中期染色体的原位杂交显示X和Y染色体配对区的重标记。在三个常染色体的亚端粒区也发现了重复序列。9号和13号染色体上似乎也存在与性染色体上类似的扩增水平。较低的拷贝数似乎存在于4号染色体上。
The pseudoautosomal region of the Mov15 mouse strain is marked by a Moloney murine leukemia provirus. The sequences flanking the Mov15 provirus were molecularly cloned and shown to consist of a tandemly repeated sequence of 31 nucleotides. Copy number variation of this repeat most likely accounts for the polymorphism in the mouse pseudoautosomal region detected with a probe from the flanking sequences. In situ hybridization to metaphase chromosomes showed heavy labeling of the pairing region of the X and Y chromosomes. The repetitive sequence was also found at the subtelomeric region of three autosomes. A similar level of amplification as the one seen on the sex chromosomes seems to be present on chromosomes 9 and 13. Lower copy numbers appear to be present on chromosome 4.