Label-free imaging of fibroblast membrane interfaces and protein signatures with vibrational infrared photothermal and phase signals
Label-free imaging of fibroblast membrane interfaces and protein signatures with vibrational infrared photothermal and phase signals
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DOI：
10.1364/boe.411888
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发表时间：
2021-01-01
影响因子：
3.4
通讯作者：
Sander, Michelle Y.
中科院分区：
文献类型：
--
作者：
Samolis, Panagis D.;Langley, Daniel;Sander, Michelle Y.
生物样品的无标记振动成像由于其结构和化学信息的集成而引起了人们的极大兴趣。振动红外光热振幅和相位信号 (VIPPS) 成像通过针对中红外指纹区域 (3 μ m - 12 μm) 中存在的生物化合物的特征共振来提供无标记化学识别。通过将吸收特征（振幅信号）的对比度与不同热特性（锁定相位信号）的灵敏检测相结合，演示了亚细胞特征的高对比度成像以及嵌入富含胶原蛋白的细胞外基质中的未标记和标记成纤维细胞中蛋白质二级结构的化学鉴定。我们提出，纳米尺寸细胞膜的可检测性增强至远低于光学衍射极限，因为发现膜充当热障。 VIPPS 提供了化学成像和热扩散表征的新颖组合，为细胞模型和组织的无标记成像以及细胞内热动力学研究铺平了道路。 (C) 2020 年美国光学学会根据 OSA 开放获取出版协议的条款
Label-free vibrational imaging of biological samples has attracted significant interest due to its integration of structural and chemical information. Vibrational infrared photothermal amplitude and phase signal (VIPPS) imaging provide label-free chemical identification by targeting the characteristic resonances of biological compounds that are present in the midinfrared fingerprint region (3 mu m - 12 mu m). High contrast imaging of subcellular features and chemical identification of protein secondary structures in unlabeled and labeled fibroblast cells embedded in a collagen-rich extracellular matrix is demonstrated by combining contrast from absorption signatures (amplitude signals) with sensitive detection of different heat properties (lock-in phase signals). We present that the detectability of nano-sized cell membranes is enhanced to well below the optical diffraction limit since the membranes are found to act as thermal barriers. VIPPS offers a novel combination of chemical imaging and thermal diffusion characterization that paves the way towards label-free imaging of cell models and tissues as well as the study of intracellular heat dynamics. (C) 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement