Analysis of 43 kb of the Chlorella virus PBCV-1 330-kb genome: map positions 45 to 88.
Analysis of 43 kb of the Chlorella virus PBCV-1 330-kb genome: map positions 45 to 88.
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小球藻病毒 PBCV-1 330-kb 基因组的 43 kb 分析：图谱位置 45 至 88。
DOI：
10.1006/viro.1995.1462
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
VanEtten,JL
中科院分区：
文献类型：
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作者：
Li,Y;Lu,Z;Burbank,DE;Kutish,GF;Rock,DL;VanEtten,JL
对330kb小球藻PBCV-1基因组左端（45 ~ 88kb）的43kb DNA进行测序和分析。共鉴定出86个大于65个密码子的开放阅读帧（orf）；47例为主要orf。这47个主要orf密集地排列在PBCV-1 DNA的两条链上。这些主要orf中有17个与序列数据库中的基因相似，包括3个涉及操纵糖的推定基因产物（葡萄糖胺合成酶、gdp - d -甘露糖脱水酶和N -乙酰氨基葡萄糖转移酶），2个转录因子，β-1,3-葡聚糖酶、天冬氨酸转氨基酶、泛素羧基末端水解酶、RNA鸟苷转移酶、核酸外切酶和解旋酶。这是首次在病毒基因组中发现这些假定的PBCV-1基因。其中一个转录因子样基因含有IB型自剪接内含子。由于先前在PBCV-1 DNA聚合酶基因中报道了剪接体加工的内含子，因此PBCV-1是已知的第一个包含两种不同类型内含子的病毒。
Forty-three kb of DNA, located at the left end (45 to 88 kb) of the 330-kb Chlorella virus PBCV-1 genome, was sequenced and analyzed. Eighty-six open reading frames (ORFs) 65 codons or longer were identified; 47 were classified as major ORFs. These 47 major ORFs are densely packed on both strands of PBCV-1 DNA. Seventeen of these major ORFs resemble genes in the sequence databases, including three putative gene products involved in manipulating sugars (glucosamine synthetase, GDP-D-mannose dehydratase, and N -acetylglucosaminyltransferase), two transcription factors, β-1,3-glucanase, aspartate transcarbamylase, ubiquitin carboxy terminal hydrolase, RNA guanyl transferase, an exonuclease, and a helicase. This is the first time some of these putative PBCV-1 genes have been found in a virus genome. One of the transcription factor-like genes contains a type IB self-splicing intron. Since a spliceosomal processed intron was reported previously in the PBCV-1 DNA polymerase gene, PBCV-1 is the first virus known to contain two different types of introns.