Mapping initiation sites for simian virus 40 DNA synthesis events in vitro.
Mapping initiation sites for simian virus 40 DNA synthesis events in vitro.
复制标题
体外绘制猿猴病毒 40 DNA 合成事件的起始位点。
DOI：
10.1128/mcb.14.8.5043-5055.1994
复制
发表时间：
1994
影响因子：
5.3
通讯作者：
Denis,D
中科院分区：
文献类型：
--
作者：
Bullock,PA;Tevosian,S;Jones,C;Denis,D
Primer RNA-DNA, a small (~30-nucleotide) RNA-DNA hybrid molecule, was identified in recent studies of simian virus 40 DNA synthesis in vitro. The available evidence indicates that primer RNA-DNA is the product of the polymerase α-primase complex. Primer RNA-DNA is formed exclusively on lagging-strand DNA templates; it is synthesized initially in the vicinity of the simian virus 40 origin and at later times at sites progressively distal to the origin. To further characterize initiation events, template sequences encoding the 5′ ends of both primer RNA and primer DNA, formed during a 5-s pulse, have been determined. Analyses of these sequences demonstrate the existence of an initiation signal for lagging-strand synthesis. At any given position, the initiation signal is located within those template sequences encoding primer RNA, situated proximal to the nucleotide encoding the 5′ end of the RNA primer. In most instances, the sequence 5′-TTN-3′ (where N encodes the nucleotide at the 5′ end of the primer) is a feature of the initiation signal. Initiation signals are present, on average, once every 19 nucleotides. These results are discussed in terms of the mechanism of Okazaki fragment formation and possible links between prokaryotic and eukaryotic initiation events.
