Involvement of PA/plasmin system in the processing of pro-MMP-9 and in the second step of pro-MMP-2 activation
Involvement of PA/plasmin system in the processing of pro-MMP-9 and in the second step of pro-MMP-2 activation
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DOI：
10.1016/s0014-5793(97)00175-0
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发表时间：
1997-03-24
期刊：
影响因子：
3.5
通讯作者：
Foidart, JM
中科院分区：
文献类型：
--
作者：
Baramova, EN;Bajou, K;Foidart, JM
MMP2原激活是一个分两步进行的过程，其结果是：(1)由基质金属蛋白酶1活性生成的中间kDa形式；(2)成熟的62 kDa形式。在不同条件下培养的HT1080细胞中加入纤溶酶原，或在其膜制备中加入纤溶酶原，可诱导中间的基质金属蛋白酶-2完全转化为成熟的基质金属蛋白酶-2，并加工成原基质金属蛋白酶-9。纤溶酶抑制剂和抗uPA抗体可抑制基质金属蛋白酶-2的激活。这些结果为PA/纤溶酶系统参与基质金属蛋白酶-2激活的第二步提供了证据。(C)1997年欧洲生化学会联合会。
Pro-MMP2 activation is a two-step process resulting in (1) an intermediate 64 kDa form generated by the MT1-MMP activity, and (2) a mature 62 kDa form. Addition of plasminogen to HT1080 cells cultured under various conditions, or to their membrane preparation, induced a complete conversion of the intermediate MMP-2 form to the mature one, and processing of pro-MMP-9. The pro-MMP-2 activation was inhibited by plasmin inhibitors and anti-uPA antibody. These results provide evidence for involvement of the PA/plasmin system in the second step of MMP-2 activation. (C) 1997 Federation of European Biochemical Societies.