The Anti-Immobility Effect of Hyperoside on the Forced Swimming Test in Rats is Mediated by the D2-Like Receptors Activation
The Anti-Immobility Effect of Hyperoside on the Forced Swimming Test in Rats is Mediated by the D2-Like Receptors Activation
复制标题
DOI：
10.1055/s-0030-1250386
复制
发表时间：
2011-03-01
期刊：
影响因子：
2.7
通讯作者：
Kuze Rates, Stela Maris
中科院分区：
文献类型：
--
作者：
Haas, Juliana Schulte;Stolz, Eveline Dischkaln;Kuze Rates, Stela Maris
巴西南部的金丝桃属植物的粗提取物在啮齿动物中表现出镇痛和抗抑郁样作用。这些物种的化学表征表明，它们富含黄酮类化合物和间苯三酚衍生物。对金丝桃属植物中一种常见的黄酮类化合物金丝桃苷（HYP）的活性进行了研究。金丝桃苷是从H. Caprifoliatum通过色谱程序。用单剂量（10、20和40 mg/kg i. p.）没有出现毒性或体重减轻的迹象。在20和40 mg/kg i. p.剂量下，小鼠在旷场试验中的探索行为减少。在20 mg/kg i. p.下，戊巴比妥睡眠时间增加，但睡眠潜伏期未增加。在热板上没有发现活性（10和20 mg/kg i. p.）或在醋酸诱导扭体试验中（20和40 mg/kg p.o.）。然而，在小鼠和大鼠的强迫游泳试验中观察到抗抑郁样作用（小鼠中HYP 10和20 mg/kg i. p.; HYP 1.8 mg/kg/天p.o.在大鼠中）。大鼠中的抗抑郁样作用被舒必利（50 mg/kg i. p.）D2拮抗剂。总之，金丝桃苷对中枢神经系统有降压作用，对啮齿类动物有抗抑郁样作用，至少部分由多巴胺能系统介导。
The crude extracts of Hypericum species native to South Brazil showed analgesic and antidepressant-like effects in rodents. The chemical characterization of these species revealed that they are rich in flavonoids and phloroglucinol derivatives. In the present study a detailed investigation was performed on the activities of hyperoside (HYP), a common flavonoid in the genus Hypericum. Hyperoside was obtained from the aerial parts of H. caprifoliatum by chromatographic procedures. Mice treated with single doses (10, 20 and 40 mg/kg i.p.) did not present signs of toxicity or weight loss. At 20 and 40 mg/kg i.p. the mice exploratory behavior in the open field test was reduced. At 20 mg/kg i.p. the pentobarbital sleeping time increased, but not the sleeping latency. No activity was found on the hot-plate (10 and 20 mg/kg i.p.) or in the acetic acid-induced writhing test (20 and 40 mg/kg p.o.). Nevertheless, an antidepressant-like effect in the forced swimming test in mice and rats was observed (HYP 10 and 20 mg/kg i.p. in mice; HYP 1.8 mg/kg/day p.o. in rats). The antidepressant-like effect in rats was prevented by the administration of sulpiride (50 mg/kg i.p.) a D2 antagonist. In conclusion, hyperoside was found to present a depressor effect on the central nervous system as well as an antidepressant-like effect in rodents which is, at least in part, mediated by the dopaminergic system.