Systematic functional analysis of the Caenorhabditis elegans genome using RNAi
Systematic functional analysis of the Caenorhabditis elegans genome using RNAi
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DOI：
10.1038/nature01278
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发表时间：
2003-01-16
期刊：
影响因子：
64.8
通讯作者：
Ahringer, J
中科院分区：
文献类型：
--
作者：
Kamath, RS;Fraser, AG;Ahringer, J
生物学家目前面临的一个主要挑战是如何将生物体的完整DNA序列与其发育和行为联系起来。大规模的靶向缺失已经成功地定义了单细胞酵母酿酒酵母中的基因功能，但类似的分析尚未在动物中进行。在此，我们描述了使用RNA干扰来抑制19，427个预测的C.优美的我们确定了1,722个基因的突变表型，其中约三分之二以前与表型无关。我们发现，类似功能的基因聚集在不同的，多个染色体区域的个体，在这些地区的基因往往共享转录谱。我们得到的数据集和可重复使用的16,757个细菌克隆的RNAi文库将有助于系统地分析C.优雅的。
A principal challenge currently facing biologists is how to connect the complete DNA sequence of an organism to its development and behaviour. Large-scale targeted-deletions have been successful in defining gene functions in the single-celled yeast Saccharomyces cerevisiae, but comparable analyses have yet to be performed in an animal. Here we describe the use of RNA interference to inhibit the function of similar to86% of the 19,427 predicted genes of C. elegans. We identified mutant phenotypes for 1,722 genes, about two-thirds of which were not previously associated with a phenotype. We find that genes of similar functions are clustered in distinct, multi-megabase regions of individual chromosomes; genes in these regions tend to share transcriptional profiles. Our resulting data set and reusable RNAi library of 16,757 bacterial clones will facilitate systematic analyses of the connections among gene sequence, chromosomal location and gene function in C. elegans.