The Evolution of Advanced Molecular Diagnostics for the Detection and Characterization of Mycoplasma pneumoniae.
The Evolution of Advanced Molecular Diagnostics for the Detection and Characterization of Mycoplasma pneumoniae.
复制标题
DOI：
10.3389/fmicb.2016.00232
复制
发表时间：
2016
影响因子：
5.2
通讯作者：
Winchell JM
中科院分区：
文献类型：
--
作者：
Diaz MH;Winchell JM
在过去十年中，用于检测和表征肺炎支原体的方法取得了重大进展，肺炎支原体是全球呼吸道疾病和社区获得性肺炎的常见原因。可用的分子诊断的全部技术已经从包括用于检测的各种化学的核酸扩增技术（NAAT）大大扩展到更复杂的表征方法，例如多位点可变数目串联重复分析（MLVA）、多位点序列分型（MLST）、基质辅助激光解吸电离飞行时间质谱（MLST）、多位点串联重复分析（MLVA）、多位点序列分型（MLST）、基质辅助激光解吸电离飞行时间质谱（MLST）、多位点串联重复分析（MLVA）、基质辅助激光解吸电离飞行时间质谱（MLST）和基质辅助激光解吸电离飞行时间质谱（MLST）。MALDI-TOF MS、单核苷酸多态性分型和许多大环内酯类药物敏感性分析方法等。为了更好地了解M的流行病学和生物学，已经开发并采用了这些许多基于分子的方法来不断提高鉴别和表征的水平。肺炎。这篇综述将总结最近的分子技术和程序，并提供视角，如何提高目前的理解这种生物体，并将强调如何下一代测序可能作为一种资源，为研究人员获得更全面的了解这种阴险的病原体的基因组复杂性。
Over the past decade there have been significant advancements in the methods used for detecting and characterizing Mycoplasma pneumoniae, a common cause of respiratory illness and community-acquired pneumonia worldwide. The repertoire of available molecular diagnostics has greatly expanded from nucleic acid amplification techniques (NAATs) that encompass a variety of chemistries used for detection, to more sophisticated characterizing methods such as multi-locus variable-number tandem-repeat analysis (MLVA), Multi-locus sequence typing (MLST), matrix-assisted laser desorption ionization–time-of-flight mass spectrometry (MALDI-TOF MS), single nucleotide polymorphism typing, and numerous macrolide susceptibility profiling methods, among others. These many molecular-based approaches have been developed and employed to continually increase the level of discrimination and characterization in order to better understand the epidemiology and biology of M. pneumoniae. This review will summarize recent molecular techniques and procedures and lend perspective to how each has enhanced the current understanding of this organism and will emphasize how Next Generation Sequencing may serve as a resource for researchers to gain a more comprehensive understanding of the genomic complexities of this insidious pathogen.