Interacting cell populations affecting granulopoietic colony formation by normal and leukemic human marrow cells.
Interacting cell populations affecting granulopoietic colony formation by normal and leukemic human marrow cells.
复制标题
相互作用的细胞群影响正常和白血病人骨髓细胞的粒细胞集落形成。
作者：
H. Messner;J. Till;E. McCulloch
28例非白血病个体的骨髓通过粘附玻璃或塑料分离成非粘附（NA）和粘附（NA）两组。与未分离的骨髓悬浮液相比，NA群体在培养过程中更依赖于添加的集落刺激活性（CSA）来形成集落，因此可以用于CSA检测。定量重建方法用于检测csa生成细胞。无论是增加辐照未分离骨髓的数量，还是从不同数量的骨髓细胞中获得的贴壁细胞，都被用来恢复NA群体的集落形成效率。在治疗前对4例急性白血病患者应用csa生成细胞的测定程序。在所有四个例子中，csa产生细胞的缺陷被证实。
Marrow from 28 nonleukemic individuals was separated by adherence to glass or plastic into nonadherent (NA) and adherent populations. The NA populations were found to be more dependent for colony formation in culture on added colonystimulating activity (CSA) than unseparated marrow suspensions, and therefore proved useful for CSA assays. Quantitative reconstitution procedures were used to assay CSA-producing cells. Either increasing numbers of irradiated unseparated marrow, or adherent cells derived from varying numbers of marrow cells, were used to restore colony-forming efficiency to NA populations. Assay procedures for CSA-producing cells were applied to four patients with acute leukemia prior to treatment. In all four instances, a defect in CSA-producing cells was demonstrated.