CRM197-conjugated serogroup C meningococcal capsular polysaccharide, but not the native polysaccharide, induces persistent antigen-specific memory B cells
CRM197-conjugated serogroup C meningococcal capsular polysaccharide, but not the native polysaccharide, induces persistent antigen-specific memory B cells
复制标题
DOI：
10.1182/blood-2006-01-009282
复制
发表时间：
2006-10-15
期刊：
影响因子：
20.3
通讯作者：
Pollard, Andrew J.
中科院分区：
文献类型：
--
作者：
Kelly, Dominic F.;Snape, Mathew D.;Pollard, Andrew J.
脑膜炎奈瑟菌是儿童细菌性脑膜炎和败血症的主要原因之一。含有来自生物体的纯化多糖胶囊（一种不依赖于T细胞的抗原）的疫苗已经存在了几十年，但似乎没有提供婴儿期保护或通过抗体应答测量的免疫记忆。相比之下，T细胞依赖性血清群C蛋白-多糖结合疫苗从婴儿期起保护免受血清群C脑膜炎球菌病的侵害，并引发免疫记忆。我们比较了浆细胞和记忆B细胞反应的大小和动力学脑膜炎球菌普通多糖疫苗和血清群C糖缀合物疫苗在青少年先前引发的结合疫苗。两种疫苗的浆细胞动力学相似，但糖缀合物的反应幅度更大。与糖缀合物疫苗相反，普通多糖疫苗不诱导持续的免疫球蛋白G（IgG）记忆B细胞应答。这是第一项直接表明血清群C脑膜炎球菌糖缀合物疫苗诱导记忆B细胞持续产生而普通多糖疫苗不诱导的研究，支持使用缀合物疫苗进行持续的群体保护。免疫后外周血记忆B细胞反应的检测可能是一个有用的新疫苗评价过程中成功诱导免疫记忆的签名。
Neisseria meningitidis is one of the leading causes of bacterial meningitis and septicemia in children. Vaccines containing the purified polysaccharide capsule from the organism, a T cell-independent antigen, have been available for decades but do not appear to provide protection in infancy or immunologic memory as measured by antibody responses. By contrast, T cell-dependent serogroup C protein-polysaccharide conjugate vaccines protect against serogroup C meningococcal disease from infancy onward and prime for immunologic memory. We compared the magnitude and kinetics of plasma cell and memory B-cell responses to a meningococcal plain polysaccharide vaccine and a serogroup C glycoconjugate vaccine in adolescents previously primed with the conjugate vaccine. Plasma cell kinetics were similar for both vaccines, though the magnitude of the response was greater for the glycoconjugate. In contrast to the glycoconjugate vaccine, the plain polysaccharide vaccine did not induce a persistent immunoglobulin G (IgG) memory B-cell response. This is the first study to directly show that serogroup C meningococcal glycoconjugate vaccines induce persistent production of memory B cells and that plain polysaccharide vaccines do not, supporting the use of the conjugate vaccine for sustained population protection. Detection of peripheral blood memory B-cell responses after vaccination may be a useful signature of successful induction of immunologic memory during novel vaccine evaluation.