TAXONOMIC STUDIES ON DEBARYOMYCES HANSENII (ZOPF) LODDER ET KREGER-VAN RIJ AND RELATED SPECIES.

TAXONOMIC STUDIES ON DEBARYOMYCES HANSENII (ZOPF) LODDER ET KREGER-VAN RIJ AND RELATED SPECIES.
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汉森德巴利酵母 (ZOPF) Lodder 等 Kreger-Van RIJ 及相关物种的分类学研究。

DOI:
10.2323/jgam.31.49
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发表时间:
1985
影响因子:
1.2
通讯作者:
Motofumi Suzuki
Motofumi Suzuki
中科院分区:
生物学4区
文献类型:
--
作者:
T. Nakase;Motofumi Suzuki

文献摘要

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采用DNA碱基组成、DNA-DNA杂交、泛素系统、甘露聚糖质子磁共振谱和血清学等化学分类学方法对汉氏酵母(Debaryomyces hansenii, ZOPF) Lodder et Kregervan Rij及其近缘种酵母进行了分类研究。根据DNA-DNA杂交实验,将50株菌株分为I、II、III 3组。这些群体被认为代表了三个不同的物种。类群I由Ia和Ib两个亚群组成,它们被认为代表了两个不同的品种。碱法提取甘露聚糖和细胞表面抗原的质子磁共振谱也对这些类群进行了表征,但除了酵母分类常用的分类标准外,它们不能通过DNA碱基组成和泛醌系统进行区分。集团是由34株包括的类型或真实的菌株d . hansenii d . nicotianae d nicotianae var.很小,d . kloeckeri d kloeckeri var.专业,d . tyrocola d . gruetzii d . matruchoti d matruchoti var. cesarii, d . hildegaardi d . guilliermondii d .味噌球拟酵母属westerdijkii, t . famata, t .轻微。Ib组共6株,分别为亚球形假丝酵母、福山假丝酵母、阿泽毕赤酵母和flareeri假丝酵母。II组包括尼泊尔血吸虫、cavensis和日本血吸虫的型株或原产株4株。第三组包括6株念珠菌型菌株。
Taxonomic studies on Debaryomyces hansenii (ZOPF) Lodder et Kregervan Rij and related yeast species were carried out using chemotaxonomic methods including DNA base composition, DNA-DNA hybridization, ubiquinone system, proton magnetic resonance spectrum of mannan, and serological methods. The 50 strains employed were classified into 3 groups, I, II, and III, based on DNA-DNA hybridization experiments. These groups were considered to represent three distinct species. Group I consisted of two subgroups, Ia and Ib, which were considered to represent two distinct varieties. These groups were also characterized by proton magnetic resonance spectra of alkali-extracted mannans and cell surface antigens, however, they could not be discriminated by DNA base composition and ubiquinone systems, in addition to the taxonomic criteria commonly employed in yeast taxonomy. Group Ia comprised 34 strains including the type or authentic strains of D. hansenii, D. nicotianae, D. nicotianae var. minor, D. kloeckeri, D. kloeckeri var. major, D. tyrocola, D. gruetzii, D. matruchoti, D. matruchoti var. cesarii, D. hildegaardi, D. guilliermondii, D. miso, Torulopsis westerdijkii, T. famata, and T. minor. Group Ib comprised 6 strains including the type or authentic strains Of D. subglobosus, D. fukuyamaensis, Pichia adzetii, and Candida flareri. Group II comprised 4 strains including the type or authentic strains of D. nepalensis, D. cavensis, and D. japonicus. Group III comprised 6 strains including the type strain of T. candida.