Preliminary Trials on the Effect of Lighting for the Population Growth of the Rotifer, Brachionus plicatilis
Preliminary Trials on the Effect of Lighting for the Population Growth of the Rotifer, Brachionus plicatilis
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光照对褶皱臂尾轮虫种群生长影响的初步试验
DOI：
10.6090/jarq.42.131
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发表时间：
2008
影响因子：
0.4
通讯作者：
Kenji Tanaka
中科院分区：
文献类型：
--
作者：
T. Yoshimatsu;T. Higuchi;Y. Hamasaki;Kenji Tanaka
以淡水小球藻为饵料，从维生素B 12（VB 12）的产生方面初步研究了光照对轮虫养殖水体的影响。在25°C、25 psu海水中，在光照和非光照条件下（L：D = 13：11和L：D= 0：24），对海洋轮虫褶皱臂尾轮虫（Brachionus plicatilis）进行了5天的分批培养。分别以富含VB12和不含VB12的小球藻（Chlorella vulgaris）为饵料，添加或不添加钴化合物（CoSO4·7H2O）。当投喂富含VB12的小球藻时，光照条件下轮虫的种群增长比完全黑暗条件下的好.另一方面，当使用不含VB12的小球藻时，在光照和黑暗组之间没有观察到种群增长的差异。然而，如果向无VB 12的小球藻中添加钴化合物，则光照组显示出比未添加组和黑暗组更高的种群增长，并且从槽中检测到的VB 12的量比来自其他组的量高得多。除此之外，即使在喂食不含VB12的小球藻而不补充Co的组中，当它们被点亮时，也观察到少量VB12的每日增加。从这些实验结果可以看出，光照对轮虫的种群增长起着重要的作用，在轮虫培养水中添加Co化合物可以提高轮虫的繁殖性能，这是由于光照促进了轮虫VB12的产生。
The effect of lighting to rotifer culture water was investigated preliminarily using freshwater Chlorella as food in terms of vitamin B 12 (VB 12 ) production. The marine rotifer Brachionus plicatilis was batch-cultured for five days at 25°C in 25 psu seawater with or without lighting (L:D = 13:11 and L:D= 0:24). Rotifers were fed VB 12 -enriched or VB 12 -flee Chlorella vulgaris with or without cobalt compound supplementation (cobalt (II) sulfate heptahydrate: CoSO 4 ·7H 2 O). When VB 12 -enriched Chlorella was fed, rotifer with lighting showed better population growth than in complete dark. On the other hand, no difference was observed in the population growth between light and dark groups when VB 12 -free Chlorella was used. Nevertheless if the cobalt compound was supplemented to VB 12 -free Chlorella, the light group showed higher population growth than non-supplemented groups and dark groups, and a much higher amount of VB 12 was detected from the tanks than those from other groups. In addition to that, even in the group fed VB 12 -free Chlorella without Co supplementation a daily increase of a small amount of VB 12 was observed when they were lit up. From these experimental results, we can conclude that lighting plays an important role for the population growth of rotifers and Co compound supplementation to rotifer culture water promotes the reproduction performance of rotifers due to the enhanced production of VB 12 by lighting.