Regulation of human and mouse oocyte maturation in vitro with 6-dimethylaminopurine
Regulation of human and mouse oocyte maturation in vitro with 6-dimethylaminopurine
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DOI：
10.1093/humrep/15.2.379
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发表时间：
2000-02-01
影响因子：
6.1
通讯作者：
Trounson, AO
中科院分区：
文献类型：
--
作者：
Anderiesz, C;Fong, CY;Trounson, AO
据推测，由于卵母细胞从小直径卵泡中恢复而导致的卵母细胞生长期的过早缩短可能是与体外成熟相关的发育异常的原因。6-二甲基氨基嘌呤（DMAP）通过抑制小鼠和人卵母细胞的生殖泡破裂，人工延长卵母细胞体外生长的前成熟期。DMAP抑制小鼠和人卵母细胞的减数分裂成熟，并且这种抑制是完全可逆的。DMAP撤回后，小鼠卵母细胞中的极体排出时间加快;然而，人卵母细胞中的核成熟动力学不受暴露于DMAP的影响。成熟至中期II的小鼠和人的经DMAP处理的卵母细胞表达组蛋白H1激酶活性。受精率在这两个DMAP处理和控制小鼠和人类卵母细胞是相当的，和人类胚胎发育是类似的控制和DMAP处理的卵母细胞。DMAP处理后，小鼠卵母细胞的囊胚发育率显著降低（P < 0.05）。因此，用DMAP暂时抑制激酶活性，延长成熟前生长期，并不能直接提高卵母细胞的发育能力，但为进一步研究体外减数分裂和发育相关事件提供了一个有用的工具。
It has been postulated that premature shortening of the oocyte growth phase due to the recovery of oocytes from small diameter follicles may be responsible for the developmental anomalies associated with in-vitro maturation. 6-Dimethylaminopurine (DMAP) was used to artificially lengthen the pre-maturation period of oocyte growth, in vitro, by inhibiting germinal vesicle breakdown in mouse and human oocytes. DMAP inhibited the meiotic maturation of mouse and human oocytes and the inhibition was fully reversible, The timing of polar body extrusion was accelerated in mouse oocytes following the withdrawal of DMAP; however, the kinetics of nuclear maturation in human oocytes was unaffected by exposure to DMAP, All. mouse and human DMAP-treated oocytes that matured to metaphase II expressed histone H1 kinase activity. Fertilization rates in both DMAP-treated and control mouse and human oocytes were comparable, and human embryonic development was similar in control and DMAP-treated oocytes. However, blastocyst development was significantly reduced in DMAP-treated mouse oocytes (P < 0.05), It is concluded that lengthening the pre-maturation growth phase, by temporarily inhibiting kinase activity with DMAP, does not directly improve oocyte developmental competence but provides a useful tool for further investigating meiotic and developmentally related events in vitro by manipulating meiotic resumption.