International Association for Dental Research Abstracts of Papers Presented at the Thirteenth Annual Meeting of the Japanese Division
International Association for Dental Research Abstracts of Papers Presented at the Thirteenth Annual Meeting of the Japanese Division
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国际牙科研究协会日本分部第十三届年会论文摘要
DOI：
10.1177/00220345670460010201
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发表时间：
1967
影响因子：
7.6
通讯作者：
M. Onisi
中科院分区：
文献类型：
--
作者：
M. Onisi
1965年12月5日东京医科齿科大学第13届日本分会年会论文摘要搪瓷的脂类成分S. Araya，S.佐佐木和我。Kaneko，东京医科齿科大学牙科学院生物化学系，东京。釉质中脂质成分的生物化学研究尚未完成。在对其他矿化组织中脂质与钙化过程关系的研究进行综述后，人们认为对胚胎釉质中脂质成分的研究是有意义的。牛牙釉质，这是假设在基质形成的阶段，用抹刀解剖和储存在干燥器中。将釉质在50° C下干燥24小时。称重并在40 ℃下在透析室中用0.1M乙二胺四乙酸（EDTA）溶液（pH7.0 - 7.5）脱钙。大约两个星期。在EDTA中的不溶性基质，约占牙釉质的5%，在室温下用丙酮、乙醚和氯仿-甲醇（1：3）连续提取2天。在该程序阶段的重量损失约为不溶性基质的35%。然后在60° C下用吡啶萃取残余物。观24小时£ ¬通过纸层析、薄层层析等方法对各组分中的脂类进行了鉴定。在乙醚提取的馏分中，在薄层色谱图上鉴定出四个斑点。它们与钼蓝溶液反应，其中3个茚三酮阳性。碱性水解（DAWSON，1960）该馏分显示纸色谱图上有一个含磷酸盐的茚三酮阳性主斑点和另外两个含磷酸盐的微弱斑点。酸水解结果表明，主斑含有较多的磷脂酰丝氨酸。氯仿-甲醇134级分重量最重，并被认为含有脂质，其具有唾液酸。在吡啶提取的馏分中，通过纸色谱法发现了可能具有硫酸盐的脂质。2.注射NAF和SRCL 2诱导的低矿化大鼠牙釉质的矿化模式S. Suga和M.片桐，病理学系，日本牙科大学，东京。通过单次注射NaF或SrCl_2溶液，诱导两种不同类型的釉质低矿化：（1）线性低矿化层，其遵循增量模式并出现在基质形成阶段;和（2）低矿化区，其遍布釉质的整个宽度并出现在成熟阶段。在本调查中，后者类型的釉质矿化不足进行了研究，借助显微放射照相术，四环素标记，和组织化学。推测在釉质成熟期，除邻近釉质牙本质交界处的一薄层外，整个釉质的矿化过程都是由成釉细胞控制的。低矿化区在吡啶预处理和DNFB法蛋白质染色后仍能被阿尔新蓝、高碘酸-希夫、苏丹黑B等染色，即使在矿化后期，当正常矿化的釉质不再被这些组织化学方法染色或不再被四环素标记时，仍能被四环素标记。这种类型的hypomineralization的发生似乎并不依赖于任何干扰的矿物相或干扰的沉积酸性粘多糖，中性多糖，和脂质作为矿化基质。相反，它似乎取决于
s of Papers Presented at the Thirteenth Annual Meeting of the Japanese Division TOKYO MEDICAL AND DENTAL UNIVERSITY, TOKYO, JAPAN, DECEMBER 5, 1965 Compiled by MASAO ONISI 1. LIPID CONSTITUENTS OF DEVELOPING ENAMEL.-S. Araya, S. Sasaki, and I. Kaneko, Department of Biochemistry, School of Dentistry, Tokyo Medical and Dental University, Tokyo. Biochemical study of the lipid constituents in enamel has not been done. On surveying the studies of lipids in other mineralized tissues in relation to their calcification process, it is thought of interest to investigate the lipid components of embryonic enamel. Bovine enamel, which was assumed in the stage of matrix formation, was dissected with a spatula and stored in a desiccator. Enamel was dried for 24 hours at 50° C. weighed, and decalcified in a dialysis chamber with 0.1 M ethylenediaminetetraacetic acid (EDTA) solution (pH 7.0 to 7.5) at 40 C. for about 2 weeks. The insoluble matrix in EDTA, which was approximately 5 percent of the enamel, was successively extracted with acetone, ether, and chloroform-methanol (1:3) at room temperature for 2 days. The weight loss at the stage of this procedure was approximately 35 percent of insoluble matrix. The residue was then extracted with pyridine at 60° C. for 24 hours. Lipids in each fraction were identified by paper chromatography, thin-layer chromatography, and some other methods. In the ether-extracted fraction, four spots were identified on thin-layer chromatograms. They reacted with molibdenum blue solution, and three of them were ninhydrinpositive. Alkaline hydrolysis (DAWSON, 1960) of the fraction revealed a phosphatecontaining, ninhydrin-positive main spot and two other phosphate-containing faint spots on paper chromatograms. The results of acid hydrolysis showed that the main spot contained a relatively large amount of phosphatidyl serine. The chloroform-methanol 134 fraction weighed the most and was assumed to contain lipid, which has sialic acid. In the pyridine-extracted fraction, a lipid which might have sulfate was found by paper chromatography. 2. MINERALIZATION PATTERN OF THE HYPOMINERALIZED RAT ENAMEL, INDUCED BY NAF AND SRCL2 INJECTION.-S. Suga and M. Katagiri, Department of Pathology, Nippon Dental College, Tokyo. By single injections of NaF or SrCI2 solutions, two different types of enamel hypomineralization were induced: (1) The lineal hypomineralized layer, which follows the incremental pattern and appears at the stage of matrix formation, and (2) the hypomineralized region, which is spread over the whole width of enamel and appears at the maturation stage. In the present investigation, the latter type of enamel hypomineralization was studied with the aid of microradiography, tetracycline labeling, and histochemistry. It is supposed that the process of mineralization of the whole width of enamel, except a very thin layer adjacent to amelodentinal junction, must be controlled by ameloblasts during the maturation stage. The hypomineralized region is still rather intensely stainable by Alcian blue, periodic acid-Schiff, Sudan black B after pyridin pretreatment, and DNFB method for protein and can still be labeled by tetracycline, even at the latter stage of mineralization, when the normally mineralized enamel ceases to be stained by these histochemical methods or to be labeled by tetracycline. The occurence of this type of hypomineralization does not seem to depend on any disturbances of the mineral phase or the disturbances of the deposition of acid-mucopolysaccharides, neutral polysaccharides, and lipids as mineralizing matrixes. Rather, it seems to depend on the