Diploid males and sex determination in a paper wasp, Polistes chinensis antennalis (Hymenoptera, Vespidae)
Diploid males and sex determination in a paper wasp, Polistes chinensis antennalis (Hymenoptera, Vespidae)
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纸黄蜂 Polistes chinensisennalis（膜翅目，胡蜂科）的二倍体雄性和性别决定
DOI：
10.1007/s00040-002-8289-8
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发表时间：
2002
期刊：
影响因子：
1.3
通讯作者：
J. Kojima
中科院分区：
文献类型：
--
作者：
Koji Tsuchida;N. Nagata;J. Kojima
除了帮助蜂王的生殖功能外，白蜂的工蜂种姓预计还会有另一种繁殖策略：在与第一窝蜂群出现的早期雄性蜂群交配后，成为替代王后。我们分析了自然群体OFP中早期男性的基因分型和染色体数目。触角中华草。从1993年到1995年，种群中早产雄性群体的频率为5.2%(4/77)。3年内4个群体和1999年的另一个早期产雄群体的性别比为1：1。对于1995年的一个早期产雄群体，我们对31只雌性和全部128只雄性进行了基因分型。67例女性中31例为杂合型，128例男性中69例为杂合型，其余59例为纯合子或半合子。该种群雌雄比例为67：69，与1：1的比例基本一致。1999年对一个早产雄蜂群体的雄性染色体进行了细胞学分析，结果表明，蜂王产卵的早期雄蜂有46条染色体，工蜂产卵的有23条。我们的结果表明，该物种的早期雄性是二倍体雄性，最终不育。其次，单基因座互补性别决定模型最能解释该物种的二倍体雄性繁殖。
Beyond helping the queen's reproductive function, the worker caste ofPolisteswasps is expected to have an alternative reproductive strategy; to become a replacement queen, following copulation with early males that emerge along with the first brood workers. We analyzed genotypes and chromosome numbers of the early males in a natural population ofP. chinensis antennalis. The frequency of early-male-producing colonies within a population was 5.2% (4/77) in 1993 through 1995. The numerical sex ratios in the 4 colonies for the 3 years and another early male-producing colony in 1999 were 1:1. For one early-male producing colony in 1995, we genotyped 31 females and all 128 males. All 31 genotyped females out of 67 emerged females showed heterozygosity and 69 out of 128 genotyped males were heterozygous; the remaining 59 males were homozygous or hemizygous. The ratio between the number of females and diploid males in this colony was 67:69, which is almost identical with one-to-one ratio. The cytological analysis of male chromosome for one early-male producing colony in 1999 revealed that the early males laid by a queen had 46 chromosomes, while worker laid males had 23. Our results revealed that the early males in this species are diploid males and eventually sterile. Secondly, the single locus complementary sex determination model is most plausible to explain the diploid male production in this species.