CIRCULAR DIFFERENTIAL SCATTERING CAN BE AN IMPORTANT PART OF THE CIRCULAR-DICHROISM OF MACROMOLECULES
CIRCULAR DIFFERENTIAL SCATTERING CAN BE AN IMPORTANT PART OF THE CIRCULAR-DICHROISM OF MACROMOLECULES
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DOI：
10.1073/pnas.80.12.3568
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发表时间：
1983-01-01
期刊：
影响因子：
--
通讯作者：
MAESTRE, MF
中科院分区：
文献类型：
--
作者：
BUSTAMANTE, C;TINOCO, I;MAESTRE, MF
入射的左右圆偏振光的差分散射可以对大分子的圆二色性做出重要贡献。原则上，圆偏振光的差分吸收和差分散射都有助于圆二色性，但差分散射对于尺寸大于光波长的1/20的颗粒越来越重要。散射贡献对于溶液中未聚集的蛋白质和核酸可能并不重要。它对病毒、膜和其他蛋白质-核酸复合物非常重要。在散射的吸收带之外，手性颗粒，只有差分散射有助于圆二色性。微分散射的符号和大小与粒子的相对取向和散射单元之间的距离定量相关。圆微分散射的解释依赖于一个简单的，经典的方法。因此，在理解测量的圆二色性时，将差分散射与颗粒（例如病毒）的结构相关联通常比将差分吸收与结构相关联更容易。
Differential scattering of incident left and right circularly polarized light can be an important contribution to the circular dichroism of macromolecules. In principle both differential absorption and differential scattering of circularly polarized light contribute to circular dichroism, but differential scattering is increasingly important for particles whose dimensions are greater than 1/20th the wavelength of light. The scattering contribution is probably not important for unaggregated proteins and nucleic acids in solution. It can be very important for viruses, membranes and other protein-nucleic acid complexes. Outside the absorption bands of the scattering, chiral particle, only differential scattering contributes to the circular dichroism. The sign and magnitude of the differential scattering is quantitatively related to the relative orientations and the distances between the scattering units of the particle. The interpretation of the circular differential scattering depends on a simple, classical method. Thus, in understanding a measured circular dichroism, it often will be easier to relate the differential scattering to the structure of a particle (such as a virus) than it is to relate the differential absorption to the structure.