Human leukemia cells synthesize and secrete proteins related to platelet-derived growth factor.
Human leukemia cells synthesize and secrete proteins related to platelet-derived growth factor.
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人类白血病细胞合成并分泌与血小板衍生生长因子相关的蛋白质。
DOI：
10.1073/pnas.83.15.5526
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发表时间：
1986
影响因子：
11.1
通讯作者：
Antoniades,HN
中科院分区：
文献类型：
--
作者：
Pantazis,P;Lanfrancone,L;Pelicci,PG;Dalla-Favera,R;Antoniades,HN
培养的人白血病细胞（HL-60）合成并分泌可被人血小板衍生生长因子（PDGF）抗血清识别的蛋白质。PDGF抗血清免疫沉淀的细胞内蛋白的分子量范围为34 kDa至240 kDa。在细胞的条件培养基中也鉴定了PDGF相关蛋白。这些免疫沉淀的蛋白质中有几种是糖基化的。从白血病细胞获得的mRNA的无细胞翻译产物中免疫沉淀出46 kDa的单一蛋白质。转化蛋白p28 sis/PDGF-2的预测氨基酸序列的C端而不是N端的抗血清也免疫沉淀HL-60细胞分泌的蛋白。这些发现为白血病细胞在培养中合成和分泌PDGF样蛋白提供了直接的证据。这些蛋白似乎不是由已知的c-sis/PDGF-2基因座编码的，因为在HL-60细胞中没有检测到sis mRNA。
Human leukemia cells in culture (HL-60) synthesize and secrete proteins that are recognized by antiserum to human platelet-derived growth factor (PDGF). The molecular mass of the intracellular proteins immunoprecipitated by PDGF antiserum ranged from 34 kDa to 240 kDa. PDGF-related proteins were also identified in the conditioned medium of the cells. Several of these immunoprecipitated proteins were glycosylated. A single protein of 46 kDa was immunoprecipitated from the cell-free translation products of mRNA obtained from the leukemia cells. Antiserum to the C but not to the N terminus of the predicted amino acid sequence of the transforming protein p28sis/PDGF-2 also immunoprecipitated proteins secreted by the HL-60 cells. These findings provide a direct demonstration for the synthesis and secretion of PDGF-like proteins by leukemia cells in culture. These proteins do not appear to be coded by the known c-sis/PDGF-2 locus since no sis mRNA was detectable in the HL-60 cells.