Formalin deposition as artifact in biopsies from patients affected by a new variant of endemic pemphigus foliaceus in El Bagre, Colombia, South America
Formalin deposition as artifact in biopsies from patients affected by a new variant of endemic pemphigus foliaceus in El Bagre, Colombia, South America
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DOI：
10.1111/j.1600-0560.2009.01492.x
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发表时间：
2010-08-01
影响因子：
1.7
通讯作者：
Smoller, Bruce
中科院分区：
文献类型：
--
作者：
Velez, Ana Maria Abreu;Howard, Michael S.;Smoller, Bruce
目的：我们的目的是在这些皮肤活检中确定这种色素的性质。方法：采用常规苏木精伊红（H&E）对10例患者和10例在疫区生活的按性别、年龄和工作活动相匹配的对照进行研究。结果：未检出任何细菌或寄生生物。具体来说，我们搜索了几种热带疾病病原体作为这种色素的可能病原体。铁染色和黑色素漂白技术未能确定这种色素的病因。然后我们尝试用苦味酸去除福尔马林色素。经过苦味酸等不同酸的强力处理后，色素被去除。结论：福尔马林色素与血色素有许多相同的性质。在这种情况下，作者建议使用中性缓冲福尔马林来防止福尔马林色素的形成，特别是在极端温度和环境湿度下进行活检时长时间固定后。Abreu Velez AM, Howard MS, Restrepo-Isaza M, Smoller B.南美哥伦比亚El Bagre地区一种新型地方性叶状天疱疮患者活检中福尔马林沉积的假象。
Aim: We aim to determine the nature of this pigment in these skin biopsies.Methods: We studied 10 patients and 10 controls matched by sex, age and work activity living in the endemic area by routine hematoxylin and eosin (H&E).Results: We were unable to find any bacteriological or parasitic organism. Specifically, we searched for several tropical disease agents as possible causative agents of this pigment. Iron stains and melanin pigment bleaching techniques failed to determine the etiology of this pigment. We then tried the removal of formalin pigment using picric acid. The pigment was removed after very strong treatment with different acids including picric acid.Conclusions: Formalin pigment shares many properties with hemozoin. In this case, the authors recommend the use of neutral buffered formalin to prevent the formation of formalin pigment especially after long periods of fixation when taking biopsies under extreme temperature and environmental humidity.Abreu Velez AM, Howard MS, Restrepo-Isaza M, Smoller B. Formalin deposition as artifact in biopsies from patients affected by a new variant of endemic pemphigus foliaceus in El Bagre, Colombia, South America.