METABOLISM OF ALPHA-TERPINOL BY PSEUDOMONAS-INCOGNITA
METABOLISM OF ALPHA-TERPINOL BY PSEUDOMONAS-INCOGNITA
复制标题
DOI：
10.1139/m84-228
复制
发表时间：
1984-01-01
影响因子：
2.8
通讯作者：
RENGANATHAN, V
中科院分区：
文献类型：
--
作者：
MADYASTHA, KM;RENGANATHAN, V
Details of the metabolism of .alpha.-terpineol by P. incognita are presented. Degradation of .alpha.-terpineol by this organism resulted in the formation of a number of acidic and neutral metabolites. Among the acidic metabolites, .beta.-isopropyl pimelic acid, 1-hydroxy-4-isopropenyl-cyclohexane-1-carboxylic acid, 8-hydroxycumic acid, oleuropeic acid, cumic acid and p-isopropenyl benzoic acid were identified. Neutral metabolites identified were limonene, p-cymene-8-ol, 2-hydroxycineole and uroterpenol. Cell-free extracts prepared from .alpha.-terpineol adapted cells convert .alpha.-terpineol, p-cymene-8-ol and limonene to oleuropeic acid, 8-hydroxycumic acid and perillic acid, respectively, in the presence of NADH. The same cell-free extract contained NAD+-specific dehydrogenase(s) which converted oleuropyl alcohol, p-cymene-7,8-diol and perillyl alcohol to their corresponding 7-carboxy acids. On the basis of various metabolites isolated from the culture medium, together with the supporting evidence obtained from enzymatic and growth studies, it appears that P. incognita degrades .alpha.-terpineol by at least 3 different routes. While one of the pathways seems to operate via oleuropeic acid, a second may be initiated through the aromatization of .alpha.-terpineol. The third pathway may involve the formation of limonene from .alpha.-terpineol and its further metabolism.
