Antimicrobial Susceptibility Testing: A Review of General Principles and Contemporary Practices
Antimicrobial Susceptibility Testing: A Review of General Principles and Contemporary Practices
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DOI：
10.1086/647952
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发表时间：
2009-12-01
影响因子：
11.8
通讯作者：
Ferraro, Mary Jane
中科院分区：
文献类型：
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作者：
Jorgensen, James H.;Ferraro, Mary Jane
临床微生物学实验室的一项重要任务是对重要细菌分离株进行抗菌药物敏感性测试。测试的目的是检测常见病原体中可能的耐药性，并确保对特定感染所选药物的敏感性。最广泛使用的测试方法包括肉汤微量稀释或使用市售材料和设备的快速自动化仪器方法。提供灵活性和可能节省成本的手动方法包括圆盘扩散和梯度扩散方法。每种方法都有优点和缺点，包括可以通过该方法准确测试的生物体。一些方法提供定量结果（例如，最低抑制浓度），并且所有方法都使用敏感、中间或耐药类别提供定性评估。一般来说，当前的测试方法可以准确检测常见的抗菌药物耐药机制。然而，新的或正在出现的耐药机制需要对每种测试方法准确检测耐药性的能力始终保持警惕。
An important task of the clinical microbiology laboratory is the performance of antimicrobial susceptibility testing of significant bacterial isolates. The goals of testing are to detect possible drug resistance in common pathogens and to assure susceptibility to drugs of choice for particular infections. The most widely used testing methods include broth microdilution or rapid automated instrument methods that use commercially marketed materials and devices. Manual methods that provide flexibility and possible cost savings include the disk diffusion and gradient diffusion methods. Each method has strengths and weaknesses, including organisms that may be accurately tested by the method. Some methods provide quantitative results (eg, minimum inhibitory concentration), and all provide qualitative assessments using the categories susceptible, intermediate, or resistant. In general, current testing methods provide accurate detection of common antimicrobial resistance mechanisms. However, newer or emerging mechanisms of resistance require constant vigilance regarding the ability of each test method to accurately detect resistance.