Clinical relevance of cagPAI intactness in Helicobacter pylori isolates from Vietnam
Clinical relevance of cagPAI intactness in Helicobacter pylori isolates from Vietnam
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DOI：
10.1007/s10096-010-0909-z
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发表时间：
2010-06-01
影响因子：
4.5
通讯作者：
Moriyama, M.
中科院分区：
文献类型：
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作者：
Nguyen, L. T.;Uchida, T.;Moriyama, M.
本研究旨在探讨越南幽门螺杆菌cagPAI基因完整性与临床转归的关系。用聚合酶链式反应和斑点印迹法检测30个cagPAI基因的存在与否。检测幽门螺杆菌诱导的白介素8分泌和蜂鸟表型，以及幽门螺杆菌对胃上皮细胞的粘附性。同时检测所有患者的血清胃蛋白酶原1、胃蛋白酶原2和胃泌素浓度。103株越南幽门螺杆菌均存在cagPAI，其中91株cagPAI完整，12株仅含有部分cagPAI。部分cagPAI菌株的感染与消化性溃疡和慢性胃黏膜炎症的相关性低于cagPAI完整菌株的感染。部分cagPAI株几乎缺乏诱导胃细胞分泌IL-8和蜂鸟表型的能力。与完整的cagPAI株相比，它们与上皮细胞的粘附性显著降低。此外，我们首次发现cagPAI状态与感染患者血清胃蛋白酶原1和2浓度之间存在关联。CagPAI内部缺失的幽门螺杆菌菌株毒力较低，因此不太可能与严重的临床结果相关。
The purpose of this paper is to investigate the relationship between clinical outcome and the intactness of cagPAI in Helicobacter pylori strains from Vietnam. The presence or absence of 30 cagPAI genes was investigated by polymerase chain reaction (PCR) and dot-blotting. H. pylori-induced interleukin-8 secretion and hummingbird phenotype, and H. pylori adhesion to gastric epithelial cells were examined. The serum concentration of pepsinogen 1, pepsinogen 2, and gastrin was also measured in all patients. cagPAI was present in all 103 Vietnamese H. pylori isolates, of which 91 had intact cagPAI and 12 contained only a part of cagPAI. Infection with the partial cagPAI strains was less likely to be associated with peptic ulcer and chronic gastric mucosal inflammation than infection with strains possessing intact cagPAI. The partial cagPAI strains lacked almost all ability to induce interleukin-8 secretion and the hummingbird phenotype in gastric cells. Their adhesion to epithelial cells was significantly decreased in comparison with intact cagPAI strains. Moreover, for the first time, we found an association between cagPAI status and the serum concentration of pepsinogens 1 and 2 in infected patients. H. pylori strains with internal deletion within cagPAI are less virulent and, thus, less likely to be associated with severe clinical outcomes.