Effect of Electrostatic Interaction on the Leaflet-specific Lipid Diffusion in a Supported Lipid Bilayer Revealed by Fluorescence Lifetime Correlation Analysis
Effect of Electrostatic Interaction on the Leaflet-specific Lipid Diffusion in a Supported Lipid Bilayer Revealed by Fluorescence Lifetime Correlation Analysis
复制标题
荧光寿命相关分析揭示静电相互作用对支撑脂质双层中小叶特异性脂质扩散的影响
DOI：
10.1039/c9cp05833h
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发表时间：
2020
影响因子：
3.3
通讯作者：
Takuhiro Otosu and Shoichi Yamaguchi
中科院分区：
文献类型：
--
作者：
Tetsuyuki Takayama;Kota Kishi;Takuhiro Otosu;Takuma Yagasaki. Shoichi Yamaguchi;Takuhiro Otosu and Shoichi Yamaguchi;Takuhiro Otosu and Shoichi Yamaguchi
负载脂质双分子层（SLB）是分析生物膜动力学特性的重要工具。然而，固体载体对SLB中叶片特异性脂质动力学的影响仍然难以捉摸，这阻碍了SLB作为模型生物膜的进一步应用。在这里，我们通过二维荧光寿命相关光谱进行了单叶特异性脂质扩散分析，以阐明脂质头基团与玻璃表面之间的静电相互作用对SLB每个单叶中脂质扩散的影响。结果清楚地显示了静电相互作用强度与面向玻璃表面的SLB近端小叶中的脂质扩散之间的相关性。特别是，阳离子脂质与带负电的玻璃表面之间的静电吸引增强了脂质在SLB近端小叶中的扩散，这不仅对SLB中的脂质动力学，也对生物膜中的脂质动力学提供了重要的意义。
A supported lipid bilayer (SLB) is now an indispensable tool to analyze the dynamical properties of biomembranes. However, the effect of a solid support on the leaflet-specific lipid dynamics in a SLB remains elusive, which hampers the further application of the SLB as a model biomembrane. Here, we performed the leaflet-specific lipid diffusion analysis by means of two-dimensional fluorescence lifetime correlation spectroscopy to elucidate the effect of the electrostatic interaction between lipid headgroups and a glass surface on the lipid diffusion in each leaflet of the SLB. The results clearly showed the correlation between the strength of the electrostatic interaction and the lipid diffusion in the proximal leaflet of the SLB facing a glass surface. In particular, the electrostatic attraction between the cationic lipids and a negatively charged glass surface enhanced the lipid diffusion in the proximal leaflet of the SLB, providing important implications for the lipid dynamics not only in the SLB but also in biomembranes.