Peptide complexation in water
Peptide complexation in water
复制标题
肽在水中的络合
DOI：
10.1016/s0040-4039(00)78233-5
复制
发表时间：
1994
影响因子：
1.8
通讯作者：
W. Still
中科院分区：
文献类型：
--
作者：
H. Wennemers;W. Still
许多商业上可用的染料与某些酰化三肽在水中的亲水性聚乙二醇聚苯乙烯小球上具有高选择性结合。评估底物/受体结合的最常见方法之一是用有色或荧光染料标记底物或受体。这种标记被用于许多重要的生物标记技术，包括免疫荧光和基于荧光的zyxwvutsrqponmlkjihgfedcb原位杂交。最近，它被用来探测生物小分子与合成受体的结合。1无论采用哪种体系，使用标记材料进行的结合研究都假定标记的存在不会显著改变受体-底物结合。事实上，我们发现许多常见的染料将水中的多肽与它们的氨基酸组成或序列具有相当大的选择性。我们的结果表明，结合分析中的染料需要非常谨慎地选择，以避免标记诱导的伪影。他们还提出了新的结构基序，可以用作新的多肽序列选择受体的基础。
Many commercially available dyes bind certain acylated tripeptides with high selectivity on hydrophilic poly (ethylene glycol)-polystyrene beads in water.One of the most common methods for assessing substrate/receptor binding entails labeling a substrate or receptor with a colored or fluorescent dye. Such labeling is used in a number of important biological marking techniques including immunofluorescence and fluorescence-based zyxwvutsrqponmlkjihgfedcb in situ hybridization. It has recently zyxwvutsrqponmlkjihgfedcbaZYXWVUTSRQPONMLKJIHGFEDCBA been used to probe the binding of small biological molecules to synthetic receptors. 1 Whatever the system, binding studies using labeled material assume that the presence of label does not significantly alter receptor-substrate binding. In fact, we find that many common dyes associate wtth peptides in water with substantial selectivity for their amino acid composition or sequence. Our results indicate that dyes in binding assays need to be chosen with great care to avoid label-induced artifacts. They also suggest novel structural motifs that may be used as the basis of new sequence-selective receptors for peptides.