Love Wave Sensor for the Detection of Staphylococcal Enterotoxins B DNA in Liquid Phase
Love Wave Sensor for the Detection of Staphylococcal Enterotoxins B DNA in Liquid Phase
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用于检测液相中葡萄球菌肠毒素 B DNA 的 Love 波传感器
DOI：
10.1166/sl.2008.502
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发表时间：
2008-12
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本文建立了一种基于爱波生物传感器的DNA杂交技术检测遗传葡萄球菌肠毒素B （SEB）的新方法。研究了生物素-亲和素体系液相包被SEB ssDNA在爱波传感器上的固定方法。传感器的敏感膜显示实验成功，重复使用3 ~ 4次。优化了0.5 mol/L NaOH作为洗脱液，该敏感膜可沉积3周，且活化损失不大。线性检测范围为35 ~ 200 ng/mL，检测限为10 ng/mL，与文献报道相似。同时，利用love wave传感器对实际样品进行检测，定量检测结果与PCR结果一致。该传感器将成为检测产生SEB的金黄色葡萄球菌的一种高灵敏度、快速、选择性的分析工具。
A new method based on the love wave biosensor for the detection of genetic Staphylococcal enterotoxins B (SEB) employing DNA hybridization technique has been established in the paper. Immobilization method which SEB ssDNA coated on the love wave sensor by the biotin-avidin system in liquid phase was studied. The sensitive film of the sensor shows the success of the experiment reused for 3 to 4 times. 0.5 mol/L NaOH as the elution solution was optimized, and the sensitive film can be deposited for 3 weeks without much activation loss. The linear detective range was 35∼200 ng/mL and the detective limit was 10 ng/mL which was similar to the reference reports. Meanwhile, the love wave sensor was employed to real sample tests and the quantitative detection was consistent to PCR results. The love sensor will become a highly sensitive, rapid, selective analytical tool for detection of Staphylococcus aureus, which produces SEB.