The role ofmab-3in spermatogenesis and ontogenesis of pinewood nematode,Bursaphelenchus xylophilus
The role ofmab-3in spermatogenesis and ontogenesis of pinewood nematode,Bursaphelenchus xylophilus
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mab-3 在松材线虫、松材线虫的精子发生和个体发生中的作用。
DOI：
10.1002/ps.6001
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发表时间：
2020-08-13
影响因子：
4.1
通讯作者：
Hu，Jiafu
中科院分区：
文献类型：
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作者：
Zhou，Lifeng;Ma，Xinxin;Hu，Jiafu
背景松材线虫是最具破坏性的入侵物种之一，在全世界造成广泛的经济损失。 B．男女性别比松材线虫在线虫侵染中发挥重要作用。然而，人们对其性别决定的过程知之甚少。双性/mab-3相关转录因子家族在动物中高度保守，在性别决定、精子发生和个体发生中发挥着至关重要的作用。因此，我们研究了它的直系同源物 Bxy-mab-3，inB。结果Bxy-mab-3 有两个典型的保守 DNA 结合域。通过 mRNA 原位杂交，在 J2（第二阶段幼虫）、J3、J4 和雄性成虫（特别是在针状体上）中观察到了这种现象，但在卵或雌性成虫中没有观察到。 RNA-Seq 表明男性中的表达显着较高。 RNAi显示雄性成虫的体型和精子大小明显小于对照组。与此同时，几乎所有经过RNAi处理的雄性都无法与正常雌性交配，甚至有26.34%的受干扰雄性不产生精子。然而，Bxy-mab-3的RNAi对B的性别比没有影响。结论Bxy-mab-3对于精子发生、个体发生和交配行为是不可或缺的。它是一种典型的性别决定基因，在男性和女性中存在差异表达。然而，敲低 Bxy-mab-3 的表达并不能改变其他物种中的性别比例。我们的研究结果有助于更好地理解 Bxy-mab-3inB 的分子事件。 xylophilus，它通过阻断个体发育和减少线虫繁殖力来控制松材线虫病提供了有希望的线索。
BackgroundBursaphelenchus xylophilusis one of the most destructive invasive species, causing extensive economic losses worldwide. The sex ratio of female to male ofB. xylophilusplays an important role in the nematode infestation. However, little is known about the processes of its sex determination. The double sex/mab‐3‐related family of transcription factors are highly conserved in animals, playing crucial roles in sex determination, spermatogenesis and ontogenesis. We therefore investigated its orthologue,Bxy‐mab‐3, inB. xylophilus.ResultsBxy‐mab‐3has two typical conserved DNA‐binding domains. It was observed in J2 (the second‐stage of juveniles), J3, J4 and male adults (specifically on the spicules), but not in eggs or female adults via mRNAin situhybridization. RNA‐Seq indicated significantly higher expression in males. RNAi showed that the body size and sperm size of male adults were markedly smaller than those of the controls. Meanwhile, almost all the RNAi‐treated males failed to mate with the normal females, even 26.34% of interfered males did not produce sperm. However, RNAi ofBxy‐mab‐3had no effect on the sex ratio ofB. xylophilus.ConclusionBxy‐mab‐3is indispensable for spermatogenesis, ontogenesis and mating behavior. It is a typical sex‐determination gene with differential expression in males and females. However, knocking downBxy‐mab‐3expression could not alter the sex ratio as seen in other species. Our findings contribute towards a better understanding of the molecular events ofBxy‐mab‐3inB. xylophilus, which provides promising hints for control of pine wilt disease by blocking ontogenesis and decreasing nematode fecundity.