Borrelia burgdorferi BBI39 Paralogs, Targets of Protective Immunity, Reduce Pathogen Persistence Either in Hosts or in the Vector.
Borrelia burgdorferi BBI39 Paralogs, Targets of Protective Immunity, Reduce Pathogen Persistence Either in Hosts or in the Vector.
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伯氏疏螺旋体 BBI39 旁系同源物是保护性免疫的目标，可减少宿主或载体中病原体的持续存在。
DOI：
10.1093/infdis/jix036
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发表时间：
2017
期刊：
影响因子：
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通讯作者：
Pal,Utpal
中科院分区：
文献类型：
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作者：
Singh,Preeti;Verma,Deepshikha;Backstedt,BrianT;Kaur,Simarjot;Kumar,Manish;Smith,AlexisA;Sharma,Kavita;Yang,Xiuli;Azevedo,JoséF;Gomes-Solecki,Maria;Buyuktanir,Ozlem;Pal,Utpal
伯氏疏螺旋体基因组含有几个旁系同源基因家族（pgf），可编码功能未知的免疫原性蛋白。蛋白质-蛋白质相互作用试验使用的传播阻断疫苗候选人，BBA 52，作为诱饵，确定了螺旋体的相互作用的合作伙伴-pgf 54的成员，注释为BBI 39。我们表明，BBI 39是一种表面暴露的膜抗原，是在螺旋体感染的免疫原性，尽管该基因主要是在载体中转录，在宿主中只在蜱叮咬位点的瞬时表达。用BBI 39或不同的寄生虫BBI 36或其组合免疫啮齿动物会损害病原体通过载体的获得、从蜱到宿主的传播或疾病的诱导。具有杀疏螺旋体特性的高滴度BBI 39免疫球蛋白G抗体可以通过常规皮下或口服免疫产生，进一步突出了BBI 39蛋白作为新型莱姆病疫苗的用途，其可以靶向宿主或蜱中的病原体。
Borrelia burgdorferigenome harbors several paralogous gene families (pgf) that can encode immunogenic proteins of unknown function. Protein–protein interaction assays using a transmission-blocking vaccine candidate, BBA52, as bait identified an interacting partner in spirochetes—a member of pgf 54, annotated as BBI39. We show that BBI39 is a surface-exposed membrane antigen that is immunogenic during spirochete infection, despite the gene being primarily transcribed in the vector with a transient expression in the host only at tick-bite sites. Immunization of rodents with BBI39, or a diverse paralog, BBI36, or their combination impaired pathogen acquisition by the vector, transmission from ticks to hosts, or induction of disease. High-titer BBI39 immunoglobulin G antibodies, which have borreliacidal properties, could be generated through routine subcutaneous or oral immunization, further highlighting use of BBI39 proteins as novel Lyme disease vaccines that can target pathogens in the host or in ticks.