Tumor necrosis factor-α-induced adipose-related protein (TIARP), a cell-surface protein that is highly induced by tumor necrosis factor-α and adipose conversion
Tumor necrosis factor-α-induced adipose-related protein (TIARP), a cell-surface protein that is highly induced by tumor necrosis factor-α and adipose conversion
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DOI：
10.1074/jbc.m105726200
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发表时间：
2001-09-07
影响因子：
4.8
通讯作者：
Chambaut-Guérin, AM
中科院分区：
文献类型：
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作者：
Moldes, M;Lasnier, F;Chambaut-Guérin, AM
肿瘤坏死因子-α（TNF-α）参与在两种相反的代谢情况下发现的生理和生物异常：恶病质和肥胖。为了鉴定能够介导TNF α对脂肪细胞代谢和发育的影响的新基因和蛋白质，我们使用了差异显示技术，比较了3 T3-L1细胞暴露于或不暴露于细胞因子。我们已经分离出一种新的脂肪cDNA编码的TNF α诱导的470个氨基酸的蛋白质，称为TIARP，与6个假定的跨膜区两侧的一个大的氨基末端和一个短的羧基末端结构域，结构让人想起通道和转运蛋白。细胞因子反应性需要致力于分化过程。分化过程本身伴随着TIARP m-RNA转录物的急剧出现，与质膜上蛋白质的表达平行。转录本在白色和棕色脂肪组织中以高水平存在，并且在肝脏、肾脏、心脏和骨骼肌中也可检测到。尽管TIARP的生物学功能目前尚不清楚，但其在脂肪转化期间和作为主要位于细胞表面的跨膜蛋白响应TNF α暴露的表达模式表明，TIARP可能参与脂肪细胞发育和代谢，并作为通道或转运蛋白介导TNF α对脂肪细胞的某些作用。
Tumor necrosis factor-alpha (TNF alpha) is involved in the physiological and biological abnormalities found in two opposite metabolic situations: cachexia and obesity. In an attempt to identify novel genes and proteins that could mediate the effects of TNFa alpha on adipocyte metabolism, and development, we have used a differential display technique comparing 3T3-L1 cells exposed or not to the cytokine. We have isolated a novel adipose cDNA encoding a TNF alpha -inducible 470-amino acid protein termed TIARP, with six putative transmembrane regions flanked by a large amino-terminal and a short carboxyl-terminal domain, a structure reminiscent of channel and transporter proteins. Commitment into the differentiation process is required for cytokine responsiveness. The differentiation process per se is accompanied by a sharp emergence of TIARP m-RNA transcripts, in parallel with the expression of the protein at the plasma membrane. Transcripts are present at high levels in white and brown adipose tissues, and are also detectable in liver, kidney, heart, and skeletal muscle. Whereas the biological function of TIARP is presently unknown, its pattern of expression during adipose conversion and in response to TNF alpha exposure as a transmembrane protein mainly located at the cell surface suggest that TIARP might participate in adipocyte development and metabolism and mediate some TNF alpha effects on the fat cell as a channel or a transporter.