Blocking Dopaminergic Signaling Soon after Learning Impairs Memory Consolidation in Guinea Pigs.
Blocking Dopaminergic Signaling Soon after Learning Impairs Memory Consolidation in Guinea Pigs.
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DOI：
10.1371/journal.pone.0135578
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Chirwa S
中科院分区：
文献类型：
--
作者：
Lee KN;Chirwa S
情景记忆的形成（即记忆的经历）需要一个称为巩固的过程，该过程涉及新皮层和海马体之间的通信。然而，这种新皮质-海马通讯的神经调节机制知之甚少。在这里，我们研究了多巴胺D1受体（D1 R）和D2受体（D2 R）介导的信号转导的参与记忆巩固使用新物体识别（NOR）测试。我们在雄性Hartley豚鼠中进行了测试，并在定制的Phenotyper家庭笼中使用Noldus的Ethovision XT软件评估了认知行为，该软件支持3点检测系统（鼻子，身体中心和后部）。我们发现，急性腹膜内注射0.25 mg/kg SCH 23390阻断D1 Rs或1.0 mg/kg舒必利阻断D2 Rs后不久收购（这涉及熟悉两个类似的对象）衰减随后的歧视新的对象时，测试后5小时的NOR测试。相比之下，用生理盐水处理的豚鼠表现出对新物体的强有力的辨别力，表明正常的操作过程巩固了记忆。这些数据表明，多巴胺能信号的参与是一个关键的收购后的因素，在调节豚鼠的记忆巩固。
Formation of episodic memories (i.e. remembered experiences) requires a process called consolidation which involves communication between the neocortex and hippocampus. However, the neuromodulatory mechanisms underlying this neocortico-hippocampal communication are poorly understood. Here, we examined the involvement of dopamine D1 receptors (D1R) and D2 receptors (D2R) mediated signaling on memory consolidation using the Novel Object Recognition (NOR) test. We conducted the tests in male Hartley guinea pigs and cognitive behaviors were assessed in customized Phenotyper home cages utilizing Ethovision XT software from Noldus enabled for the 3-point detection system (nose, center of the body, and rear). We found that acute intraperitoneal injections of either 0.25 mg/kg SCH23390 to block D1Rs or 1.0 mg/kg sulpiride to block D2Rs soon after acquisition (which involved familiarization to two similar objects) attenuated subsequent discrimination for novel objects when tested after 5-hours in the NOR test. By contrast guinea pigs treated with saline showed robust discrimination for novel objects indicating normal operational processes undergirding memory consolidation. The data suggests that involvement of dopaminergic signaling is a key post-acquisition factor in modulating memory consolidation in guinea pigs.