Basement membrane-like matrix sponge for the three-dimensional proliferation culture of differentiated retinal horizontal interneurons
Basement membrane-like matrix sponge for the three-dimensional proliferation culture of differentiated retinal horizontal interneurons
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DOI：
10.1016/j.biomaterials.2011.04.062
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发表时间：
2011-09-01
期刊：
影响因子：
14
通讯作者：
Mizusawa, Hidehiro
中科院分区：
文献类型：
--
作者：
Ajioka, Itsuki;Ichinose, Shizuko;Mizusawa, Hidehiro
由于中枢神经系统(CNS)中的神经元以三维(3D)方式形成神经元网络，因此需要一种用于分化神经元的3D增殖培养系统。虽然以前认为分化的神经元永远不会增殖，但在体内，Rb-/-；p107+/-；p130-/-(p107-Single)视网膜分化的水平中间神经元克隆性增殖而没有去分化。在本研究中，我们研制了一种基底膜样基质海绵(BM-海绵)，用于分化的水平中间神经元的三维增殖培养。P107-单个水平中间神经元，而不是其他类型的视网膜神经元，在BM-海绵中以3D方式增殖。这些中间神经元表达突触前标记，并形成突触小泡。这些数据表明，在BM-海绵中培养的p107-单个水平中间神经元在增殖的同时保持其分化特征。我们在这里描述了分化神经元的三维增殖培养系统。(C)2011爱思唯尔有限公司。保留所有权利。
As the neurons in the central nervous system (CNS) form a neuronal network in a three-dimensional (3D) manner, a 3D proliferation culture system for differentiated neurons has been desired. Although differentiated neurons were previously thought to never proliferate, differentiated horizontal interneurons of Rb-/-; p107+/-; p130-/- (p107-single) retina clonally proliferated without dedifferentiation in vivo Ill. In the present study, we developed a basement membrane-like matrix sponge (BM-sponge) for the 3D proliferation culture of differentiated horizontal interneurons. p107-single horizontal interneurons, but not other types of retinal neurons, proliferated in the BM-sponge in a 3D manner. These interneurons expressed presynaptic marker and developed synaptic vesicles. These data demonstrated that p107-single horizontal interneurons cultured in the BM-sponge proliferate while maintaining their differentiated features. We described here the 3D proliferation culture system for differentiated neurons. (C) 2011 Elsevier Ltd. All rights reserved.