Estimation of nicotine dose after low-level exposure using plasma and urine nicotine metabolites.
Estimation of nicotine dose after low-level exposure using plasma and urine nicotine metabolites.
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DOI：
10.1158/1055-9965.epi-09-1303
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发表时间：
2010-05
期刊：
影响因子：
--
通讯作者：
Jacob P 3rd
中科院分区：
文献类型：
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作者：
Benowitz NL;Dains KM;Dempsey D;Yu L;Jacob P 3rd
我们试图确定最佳血浆和尿液尼古丁代谢物，单独或联合，以估计低水平暴露后尼古丁的全身剂量。我们每天给36名不吸烟者口服100、200或400 μg的氘标记尼古丁（剂量与二手烟暴露相似，SHS） 5天，然后在24小时内以不同的间隔测量血浆和尿液尼古丁代谢物。24小时尿液中主要尼古丁代谢物的四种[可替宁+可替宁-葡萄糖醛酸盐+反式-3′-羟基可替宁+ 3hg -葡萄糖醛酸盐]或六种[也包括尼古丁+尼古丁-葡萄糖醛酸盐]的总和与尼古丁剂量的相关性最强（r = 0.96），在一天中不同时间使用经肌酐校正的尿样与这些代谢物的相关性较小（r = 0.72 - 0.80）。血浆[可替宁+反式3′羟基可替宁]与尼古丁剂量的相关性高于血浆可替宁（r = 0.82 vs 0.75）。我们的研究结果为选择生物标志物来估计低水平（SHS）烟草暴露的尼古丁剂量提供了指导。这可能也与主动吸烟有关。
We sought to determine the optimal plasma and urine nicotine metabolites, alone or in combination, to estimate the systemic dose of nicotine after low level exposure. We dosed 36 nonsmokers with 100, 200 or 400 μg deuterium-labeled nicotine (doses similar to exposure to secondhand smoke, SHS) by mouth daily for 5 days and then measured plasma and urine nicotine metabolites at various intervals over 24 hours. The strongest correlations with nicotine dose were seen for the sum of four [cotinine + cotinine-glucuronide + trans-3′-hydroxycotinine + 3HC-glucuronide] or six [ including also nicotine + nicotine-glucuronide] of the major nicotine metabolites in 24 hour urine collection (r = 0.96), with lesser correlations for these metabolites using spot urines corrected for creatinine at various times of day (r = 0.72 – 0.80). Plasma [cotinine + trans 3′ hydroxycotine] was more highly correlated with nicotine dose than plasma cotinine alone (r = 0.82 vs 0.75). Our results provide guidance for selection of biomarkers to estimate the dose of nicotine taken in low level (SHS) tobacco exposure. This is probably relevant to active smoking as well.