Distribution, fine structure, and three-dimensional innervation of lamellar corpuscles in rat plantar skin.
Distribution, fine structure, and three-dimensional innervation of lamellar corpuscles in rat plantar skin.
复制标题
大鼠足底皮肤板层小体的分布、精细结构和三维神经支配。
DOI：
10.1007/s00441-021-03525-5
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发表时间：
2021
期刊：
影响因子：
3.6
通讯作者：
Kumamoto K.
中科院分区：
文献类型：
--
作者：
Koike T;Ebara S;Tanaka S;Kase M;Hirahara Y;Hayashi S;Oe S;Nakano Y;Kitada M;Kumamoto K.
板层小体在皮肤中起机械感受器的作用，由轴突终末和由终末雪旺细胞构成的板层组成。根据组织学标准，它们可分为太平洋小体、迈斯纳小体和单纯性小体。大鼠毛乳头细胞中的板层小体已有报道，但其形态特征仍有待深入研究。在本研究中，我们分析了大鼠足底皮肤板层小体的酶活性、分布、精细结构和三维神经支配。表现出非特异性胆碱酯酶的板层小体密集分布在大鼠的足垫中，明显的皮肤隆起，特别是在顶端，每个足垫中脊的最高部分。相比之下，在其他足底皮肤区仅发现少量板层小体。板层小体被认为是由扁平的轴突终末雪旺细胞板组成，这些板层大致同心排列在真皮乳头中。这些组织学特征与单纯小体的特征相一致。此外，轴突示踪法显示，一个主干轴突支配多个简单小体。主干轴突的区域相互重叠。最后，对动物的足迹进行了分析。在暂停和行走阶段，脚垫经常与地板接触。这些结果表明，大鼠足底皮肤真皮乳头中的板层小体类型是一种简单的小体，提示它们在足底皮肤中的分布模式便于有效地感知和传递来自地面的机械刺激。
Lamellar corpuscles function as mechanoreceptors in the skin, composed of axon terminals and lamellae constructed by terminal Schwann cells. They are classified into Pacinian, Meissner, and simple corpuscles based on histological criteria. Lamellar corpuscles in rat dermal papilla cells have been reported; however, the morphological aspects have yet to be thoroughly investigated. In the present study, we analyzed the enzyme activity, distribution, fine structure, and three-dimensional innervation of lamellar corpuscles in rat plantar skin. The lamellar corpuscles exhibiting non-specific cholinesterase were densely distributed in rat footpads, evident as notable skin elevations, especially at the apex, the highest portion of the ridges in each footpad. In contrast, only a few lamellar corpuscles were found in other plantar skin areas. Lamellar corpuscle was considered composed of a flat axon terminal Schwann cell lamellae, which were roughly concentrically arranged in the dermal papilla. These histological characteristics correspond to those of the simple corpuscle. Moreover, the axon tracing method revealed that one trunk axon innervated several simple corpuscles. The territory of the trunk axons overlapped with each other. Finally, the animals’ footprints were analyzed. During the pausing and walking phases, footpads are often in contact with the floor. These results demonstrate that the type of lamellar corpuscles in the dermal papillae of rat plantar skin is a simple corpuscle and implies that their distribution pattern in the plantar skin is convenient for efficient sensing and transmission of mechanical stimuli from the ground.