Downregulation of Otx2 in the dedifferentiated RPE cells of regenerating newt retina
Downregulation of Otx2 in the dedifferentiated RPE cells of regenerating newt retina
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DOI：
10.1016/j.devbrainres.2004.11.008
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发表时间：
2005-03-22
期刊：
影响因子：
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通讯作者：
Tokunaga, F
中科院分区：
文献类型：
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作者：
Sakami, S;Hisatomi, O;Tokunaga, F
Cymops pyrrhogaster（日本普通蝾螈）从视网膜色素上皮（RPE）细胞再生神经视网膜。Otx 2是参与RPE细胞分化的转录因子。为了了解Otx 2在RPE细胞转分化过程中的作用，我们克隆了一个Cymops Otx 2？用RT-PCR、免疫组织化学和原位杂交方法检测其表达。将Otx 2的表达与增殖细胞标记物（PCNA）、RPE细胞标记物（RPE 65，CRBP）和RPE和Muller胶质细胞标记物（CRALBP）的定位进行比较。在再生早期，由色素细胞组成的2 - 3细胞层再生视网膜均匀表达Otx 2和其他标志物。在此阶段之后，4-细胞层再生视网膜由两个不同的层组成，附着于Bruch膜的色素单层（外层）和假定的神经视网膜（内层）。在细胞外层，Otx 2和CRBP表达维持不变，多数细胞PCNA表达缺失。部分细胞维持RPE 65。在内层，Otx 2，CRBP和RPE 65的表达下调，但大多数这些细胞保持PCNA表达。这些结果表明，时空调控Otx 2的表达是一致的RPE标志物。Otx 2可能在神经视网膜再生过程中RPE细胞的维持和特化中起关键作用。与RPE细胞标志物相反，CRALBP在色素层和脱色素层中均表达。这一观察结果暗示了Muller神经胶质细胞在视网膜再生的早期阶段的出现。(c)2004 Elsevier B. V.保留所有权利。
Cymops pyrrhogaster (the Japanese common newt) regenerates neural retina from retinal pigmented epithelium (RPE) cells. Otx2 is a transcription factor that is involved in RPE cell differentiation. To understand the role of Otx2 during transdifferentiation of RPE cells, we cloned a Cymops Otx2? cDNA, and explored its expression by RT-PCR, immunohistochemistry and in situ hybridization. The expression of Otx2 was compared with the localization of a proliferating cell marker (PCNA), RPE cell markers (RPE65, CRBP) and an RPE and Muller glial cell marker (CRALBP). At the early stage of regeneration, 2 to 3 cell layered regenerating retina consisting of pigmented cells uniformly expressed Otx2 and other markers. Following this stage, 4-cell layered regenerating retina consisted of two distinct layers, pigmented monolaver (the outer layer) attached to Bruch's membrane and presumptive neural retina (the inner layers). In the outer layer, Otx2 and CRBP expression seas maintained and majority of cells lost PCNA expression. Some of cells maintained RPE65. In the inner layers, expression of Otx2, CRBP and RPE65 was downregulated, but a majority of those cells maintained PCNA expression. These results indicate that spatiotemporal regulation of Otx2 expression is consistent with those of RPE markers. Otx2 may play a pivotal role in maintenance and specification of RPE cells during neural retina regeneration. In contrast to RPE cell markers, CRALBP was expressed in both the pigmented and the de-pigmented layers. This observation implicates the appearance of Muller glial cells in an early phase of regenerating retina. (c) 2004 Elsevier B.V. All rights reserved.