PREPARATION OF MULTIVESICULAR LIPOSOMES
PREPARATION OF MULTIVESICULAR LIPOSOMES
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DOI：
10.1016/0005-2736(83)90504-7
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发表时间：
1983-01-01
期刊：
影响因子：
--
通讯作者：
MARTIN, GM
中科院分区：
文献类型：
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作者：
KIM, S;TURKER, MS;MARTIN, GM
将有机溶剂从悬浮于水中的氯仿-乙醚微球中挥发出来，制备了一种新型的脂质体，称为多囊脂质体。在每个球体内有许多水滴，其中含有溶质，当溶剂挥发时，这些溶质会被困在脂质体中。制备了不同平均直径的脂质体制剂，直径从29。+-不等。10微米至5.6微米+-。1.7微米。用光学显微镜和透射电子显微镜对脂质体进行了形态表征。成功捕获到脂质体中的物质分子大小从葡萄糖到核酸不等。获得了极高的包封率(高达89%)。
A novel type of liposome, named here multivesicular liposomes, was prepared by evaporation of organic solvents from chloroform-ether spherules suspended in water. Within each spherule were numerous water droplets that contained solutes to be trapped in liposomes upon solvent evaporation. Liposome preparations of different average diameters were made, varying from 29 .+-. 10 .mu.m to 5.6 .+-. 1.7 .mu.m. The liposomes were morphologically characterized by light microscopy and transmission electron microscopy. Materials successfully trapped within the liposomes ranged in molecular size from glucose to nucleic acids. Extremely high percentages of encapsulation (up to 89%) were achieved.