DNAzyme switches for molecular computation and signal amplification
DNAzyme switches for molecular computation and signal amplification
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DOI：
10.1016/j.bios.2015.03.057
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发表时间：
2015-08-15
影响因子：
12.6
通讯作者：
Todd, Alison V.
中科院分区：
文献类型：
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作者：
Bone, Simon M.;Lima, Nicole E.;Todd, Alison V.
我们已经创造了分子开关，它由核酸切割DNA酶组成，通过与阻断寡核苷酸杂交而暂时失活。开关的独特设计提供了优于现有方法的显著优点。首先，开关被核酸切割酶激活，该核酸切割酶可以仅在特定靶分析物存在下起作用。这允许它们用作报告元件，其可以容易地适用于计算逻辑运算。其次，每个开关的激活产生活性核酸切割DNA酶作为输出，并且这允许开关彼此模块化地偶联，使得一个开关的输出充当另一个开关的输入。此外，开关是可扩展的，使得单个输入靶标可以产生多于一个活性DNAzyme输出。因此，这些特征创造了用于放大信号的手段，这赋予了未来生物传感应用的巨大潜力，其中需要检测少量的靶生物标志物。(C)2015 Elsevier B.V.版权所有。
We have created molecular switches that consist of nucleic-acid cleaving DNAzymes which are temporarily inactivated by hybridization with blocking oligonucleotides. The unique design of the switches offers significant advantages over existing methods. Firstly, the switches are activated by a nucleic acid-cleaving enzyme which can be made to function only in the presence of a specific target analyte. This allows for their use as reporter elements which can be easily adapted for use in computational logical operations. Secondly, the activation of each switch produces an active nucleic acid-cleaving DNAzyme as an output and this allows the switches to be modularly coupled to one another so that the output of one switch functions as the input of another. In addition, the switches are scalable, so that a single input target can produce more than one active DNAzyme output. These features therefore create the means for amplification of signal, which confers significant potential for future biosensing applications where detection of low quantities of target biomarkers is required. (C) 2015 Elsevier B.V. All rights reserved.