AN ESSENTIAL SACCHAROMYCES-CEREVISIAE GENE HOMOLOGOUS TO SNF2 ENCODES A HELICASE-RELATED PROTEIN IN A NEW FAMILY
AN ESSENTIAL SACCHAROMYCES-CEREVISIAE GENE HOMOLOGOUS TO SNF2 ENCODES A HELICASE-RELATED PROTEIN IN A NEW FAMILY
复制标题
DOI：
10.1128/mcb.12.4.1893
复制
发表时间：
1992-04-01
影响因子：
5.3
通讯作者：
CARLSON, M
中科院分区：
文献类型：
--
作者：
LAURENT, BC;YANG, XL;CARLSON, M
酿酒酵母SNF 2基因影响许多双链调节基因的表达，并与转录激活有关。 我们在这里报告的STH 1，SNF 2同源基因的克隆和表征。 STH 1是有丝分裂生长所必需的，在功能上与SNF 2不同。 双功能STH 1-β-半乳糖苷酶蛋白位于细胞核中。 预测的155，914-Da STH 1蛋白在661个氨基酸上与SNF 2具有72%的同一性，在另一段66个氨基酸上具有46%的同一性。 STH 1和SNF 2都含有一个假定的核苷三磷酸结合位点和类似于共有解旋酶基序的序列。 STH 1和SNF 2共享的大区域同源性在其他真核蛋白中是保守的，并且STH 1和SNF 2似乎定义了与解旋酶相关的新蛋白质家族。
The Saccharomyces cerevisiae SNF2 gene affects the expression of many diversely regulated genes and has been implicated in transcriptional activation. We report here the cloning and characterization of STH1, a gene that is homologous to SNF2. STH1 is essential for mitotic growth and is functionally distinct from SNF2. A bifunctional STH1-beta-galactosidase protein is located in the nucleus. The predicted 155,914-Da STH1 protein is 72% identical to SNF2 over 661 amino acids and 46% identical over another stretch of 66 amino acids. Both STH1 and SNF2 contain a putative nucleoside triphosphate-binding site and sequences resembling the consensus helicase motifs. The large region of homology shared by STH1 and SNF2 is conserved among other eukaryotic proteins, and STH1 and SNF2 appear to define a novel family of proteins related to helicases.