Analysis of the immunoproteome of Mycoplasma mycoides subsp mycoides small colony type reveals immunogenic homologues to other known virulence traits in related Mycoplasma species
Analysis of the immunoproteome of Mycoplasma mycoides subsp mycoides small colony type reveals immunogenic homologues to other known virulence traits in related Mycoplasma species
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DOI：
10.1016/j.vetimm.2009.04.016
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发表时间：
2009-10-15
影响因子：
1.8
通讯作者：
Gerlach, Gerald F.
中科院分区：
文献类型：
--
作者：
Jores, Joerg;Meens, Jochen;Gerlach, Gerald F.
由支原体引起的传染性牛胸膜肺炎。小菌落型（MmmSC）在发达国家已被根除，但在撒哈拉以南非洲的许多国家仍然存在。在20世纪60年代采取初步成功的控制措施之后，由于缺乏资金、兽医服务分散、牛只流动不受控制、疫苗效力不足以及目前诊断测试的敏感性，该病一直在蔓延。在这项研究中，我们使用二维聚丙烯酰胺凝胶电泳，随后对感染mmmsc的动物血清进行免疫印迹和MALDI-ToF质谱鉴定新的免疫原性蛋白，作为改进诊断和疫苗的候选分子。我们从实验感染的牛血清中鉴定出24种免疫原。此外，来自患有急性临床疾病和严重病理形态学病变的动物的血清识别出13个额外的免疫原，表明牛对CBPP的抗体反应存在差异。大多数免疫原与两种反刍动物致病菌山羊分枝杆菌的蛋白/基因序列具有显著的相似性。丝状支原体和丝状支原体。大型菌落型均属于菌类。其中三种蛋白质，即甘油-3-磷酸氧化酶、腺苷琥珀酸合成酶和甘油醛-3-磷酸脱氢酶，在其他远亲牛病原体无乳杆菌中没有明显的同源物。此外，翻译延伸因子Tu、热休克蛋白70、丙酮酸脱氢酶和fkbp型肽酰脯氨酸异构酶在其他支原体中被发现介导与宿主组织的粘附，并被血清表达和识别。这些蛋白质具有开发改进的诊断测试和可能的疫苗的潜力。(C) 2009 Elsevier B.V.版权所有
Contagious bovine pleuropneumonia (CBPP) caused by Mycoplasma mycoides subsp. mycoides small colony type (MmmSC) has been eradicated in the developed world, but it is still present in many countries of sub-Saharan Africa. After initially successful control measures in the 1960s it has been spreading due to a lack of money, fragmentation of veterinary services, uncontrolled cattle movement, insufficient vaccine efficacy and sensitivity of current diagnostic tests.In this study we used two-dimensional polyacrylamide gel electrophoresis followed by immunoblot with sera from MmmSC-infected animals and MALDI-ToF mass spectrometry to identify novel immunogenic proteins as candidate molecules for improved diagnostics and vaccines. We identified 24 immunogens recognized by pooled sera from experimentally infected cattle. Furthermore, a serum from an animal with acute clinical disease as well as severe pathomorphological lesions recognized 13 additional immunogens indicating variation in the antibody responses to CBPP amongst cattle. Most immunogens showed compelling similarity to protein/gene sequences in the two ruminant pathogens M. capricolum subsp. capricolum and M. mycoides subsp. mycoides large colony type both belonging to the mycoides cluster. Three of these proteins, namely glycerol-3-phosphate oxidase, adenylosuccinate synthase, and glyceraldehyde-3-phosphate dehydrogenase, had no compelling homologue in the other distantly related bovine pathogen M. agalactiae. In addition, translation elongation factor Tu, heat shock protein 70, pyruvate dehydrogenase, and FKBP-type peptidyl-prolyl isomerase, which have been found to mediate adhesion to host tissue in other mycoplasmas were shown to be expressed and recognized by sera. These proteins have potential for the development of improved diagnostic tests and possibly vaccines. (C) 2009 Elsevier B.V. All rights reserved.