HUMORAL IMMUNE-RESPONSES IN MURINE PREGNANCY .1. ANTI-PATERNAL ALLOANTIBODY LEVELS IN MATERNAL SERUM
HUMORAL IMMUNE-RESPONSES IN MURINE PREGNANCY .1. ANTI-PATERNAL ALLOANTIBODY LEVELS IN MATERNAL SERUM
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DOI：
10.1016/0165-0378(81)90024-3
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发表时间：
1981-01-01
影响因子：
3.4
通讯作者：
BILLINGTON, WD
中科院分区：
文献类型：
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作者：
BELL, SC;BILLINGTON, WD
用免疫珠吸附试验研究了C57BL(H-2b)雌性小鼠与CBA(H-2k)雄性小鼠交配后产生抗父系同种异体抗体的动力学和性质。首次妊娠或产后均未检测到同种异体抗体。在女性中，72%的女性在第二次怀孕时表现出体液反应，从性交后16天或17天就可以检测到，几乎所有女性在第三次怀孕时都有反应。柱层析和免疫电泳法分析表明，该同种抗体为免疫球蛋白。虽然被动转移实验显示了与实验性超免疫诱导的细胞毒抗体相似的稳定性特征，但妊娠诱导的同种抗体并不抑制补体依赖的细胞毒作用。这些发现与来自胚胎的免疫原性刺激的性质以及异基因妊娠中体液免疫反应的调节和可能的功能有关。
The kinetics and properties of anti-paternal alloantibody produced by female C57BL(H-2b) mice in response to mating with CBA(H-2k) males were investigated using an immunobead adsorption assay. No alloantibody was even detected in the 1st pregnancy or postpartum period. Of the females, 72% exhibited a humoral response in their 2nd pregnancy, detectable from day 16 or 17 post-coitum, and almost all females responded in their 3rd pregnancy. Column chromatographic and immunoelectrophoretic analysis showed that the alloantibody was IgG. Although passive transfer experiments suggested similar stability characteristics to those of cytotoxic antibody induced by experimental hyperimmunization, the pregnancy-induced alloantibody did not inhibit complement-dependent cytotoxicity. The findings are discussed in relation to the nature of the immunogenic stimulus from the conceptus and the regulation and possible function of the humoral immune response in allogeneic pregnancy.