Induction of differentiation of human myeloid leukemias: surface changes probed with monoclonal antibodies.
Induction of differentiation of human myeloid leukemias: surface changes probed with monoclonal antibodies.
复制标题
DOI：
10.1182/blood.v61.1.171.171
复制
发表时间：
1983
期刊：
影响因子：
20.3
通讯作者：
D. Ferrero;S. Pessano;G. Pagliardi;G. Rovera
中科院分区：
文献类型：
--
作者：
D. Ferrero;S. Pessano;G. Pagliardi;G. Rovera
用具有分化阶段和谱系特异性的单抗，研究了三种急性髓系白血病细胞系(HL60、ML3和KG1)在不同诱导剂(二甲基亚砜、维甲酸、12-O-十四烷基佛波醇-13-乙酸酯和淋巴细胞条件培养液中存在的因子)诱导分化过程中的表面变化。在所有的病例中，分化的表型都是有缺陷的，并且随着诱导剂和所使用的细胞系的不同而不同。结果表明，HL60对诱导剂的影响最为敏感。维甲酸诱导HL60细胞向粒细胞分化的能力优于DMSO，TPA诱导HL60细胞向单核细胞分化的能力优于TPA。这些发现表明，急性髓系白血病的分化障碍是异质性的，每个细胞系具有不同的表型特征，这些表型特征决定了用给定的诱导剂获得的分化程度。这些结果还表明，对于每个特定的白血病株，使用更合适的诱导剂可以提高体外分化反应的程度。
The surface changes occurring in three acute myeloid leukemia cell lines (HL60, ML3, and KG1) induced to differentiate by a variety of agents (dimethylsulfoxide, retinoic acid, 12-O-tetradecanoylphorbol-13-acetate, and factors present in lymphocyte conditioned medium) were probed using monoclonal antibodies that are differentiation stage- and lineage-specific. In all cases, the differentiated phenotype was defective and varied with the inducing agent and the cell line used. HL60 proved to be the most sensitive to the effect of the inducers. Retinoic acid was better than DMSO, and TPA was better than the medium factors in the ability to induce granulocytic and monocytic differentiation, respectively, in HL60 cells. These findings indicate that the differentiation block in acute myeloid leukemias is heterogeneous and that each cell line has different phenotypic characteristics that are responsible for the extent of differentiation obtained with a given inducer. These results also suggest that the extent of the differentiation response in vitro may be improved by the use of more suitable inducers for each specific leukemic line.