Molecular validation of a multiple-allele recessive genic male sterility locus (BnRf) in Brassica napus L.
Molecular validation of a multiple-allele recessive genic male sterility locus (BnRf) in Brassica napus L.
复制标题
甘蓝型油菜多等位基因隐性雄性不育位点（BnRf）的分子验证。
DOI：
10.1007/s11032-012-9708-9
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发表时间：
2012-08-01
影响因子：
3.1
通讯作者：
Yang, Guangsheng
中科院分区：
文献类型：
--
作者：
Dong, Faming;Hong, Dengfeng;Yang, Guangsheng
隐性雄性不育（RGMS）系9012AB已在中国成功用于油菜杂交生产。这种雄性不育此前被认为是由三个独立基因（Bnms3、Bnms4 和 BnRf）控制的。在这里，我们初步尝试定位 BnMs4 基因座并开发可行的分子标记以应用于实际油菜育种。然而，我们发现三个序列特征扩增区域标记和五个被鉴定为与 BnMs4 连锁的简单序列重复标记也与 BnRf 遗传相关，表明这两个基因座可能共定位。此外，我们证明与 BnRf 紧密连锁或共分离的四个基于内含子的多态性标记也可以映射到 BnMs4，遗传距离范围为 0.054 至 0.594 cM。最后，BnRf 和 BnMs4 周围遗传图谱的整合允许将两个基因座物理限制为约 50 kb 的 DNA 片段。系统基因测试还提供了证据表明候选 BnMs4 基因座与 BnRf 基因座等位。这些结果证实了9012A不育遗传模型的重大修改：具体来说，这种雄性不育本质上是由两个基因座（BnMs3和BnRf）控制的，而之前指定的BnMs4基因座（以下称为BnRfa）只是BnRf的一个等位基因，除了BnRfb（来自9012A的等位基因）和BnRfc（来自9012A的等位基因）之外。 临时维护者），主导关系为 BnRfa > BnRfb > BnRfc。该遗传模型将简化与该 RGMS 品系相关的育种过程，特别是使用此处鉴定的 BnRf 等位基因特异性分子标记。
The recessive genic male sterility (RGMS) line 9012AB has been used successfully for rapeseed hybrid production in China. This male sterility was previously thought to be controlled by three independent genes (Bnms3, Bnms4, and BnRf). Here, we initially attempted to locate the BnMs4 locus and develop feasible molecular markers for application in practical rapeseed breeding. However, we found that three sequence characterized amplified region markers and five simple sequence repeat markers identified as linked to BnMs4 were also genetically associated with BnRf, suggesting the possible co-localization of these two loci. Moreover, we proved that four intron-based polymorphism markers tightly linked or co-segregated with BnRf could also be mapped to BnMs4 with a genetic distance ranging from 0.054 to 0.594 cM. Finally, integration of genetic maps around BnRf and BnMs4 allows for the physical restriction of both loci to a DNA fragment of about 50 kb. Systematic genetic tests also provided evidence that the candidate BnMs4 locus was allelic to the BnRf locus. These results confirmed a major modification of the sterility inheritance model in 9012A: specifically, that this male sterility was essentially controlled by two loci (BnMs3 and BnRf), whereas the previously designated BnMs4 locus (hereafter designated as BnRfa) was just one allele of BnRf in addition to BnRfb (the allele from 9012A) and BnRfc (the allele from temporary maintainer), with a dominance relationship of BnRfa > BnRfb > BnRfc. This inheritance model will simplify the breeding process involved with this RGMS line, especially with the BnRf allele-specific molecular markers identified here.