Interspecific Relationship and Genetic Diversity of Zoysiagrass Revealed by SSR Markers
Interspecific Relationship and Genetic Diversity of Zoysiagrass Revealed by SSR Markers
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发表时间：
2008
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通讯作者：
Xuan Jiping
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作者：
Xuan Jiping
对隶属于5个种和1个突变种的96份结缕草种质资源的遗传多样性和种间亲缘关系进行了分析。结果表明：(1)29对SSR引物共扩增出282条带，其中272条具有多态性，多态率为96.45%，Nei‘s基因多样性指数(H)为0.2203，Shannon多样性指数(I)为0.3504；(2)96份结缕草种质间的遗传相似系数在0.5922~0.9362之间，平均为0.7811。结缕草与结缕草之间、结缕草与细叶结缕草之间的遗传相似系数较高，其中大叶结缕草(Z010)和结缕草(Z122)的遗传相似系数较低。结缕草、结缕草和结缕草的遗传相似系数分别为0.5922~0.9362、0.6879~0.9078和0.6738~0.8582(3)聚类分析表明，96份结缕草种质间的遗传距离和亲缘关系清晰，可将96份结缕草种质分为6大类群。第一类群包括日本结缕草、中华结缕草和部分结缕草种质，占种质总数的90.6%；马尾草和细叶结缕草的部分种质聚为一类，其中大叶结缕草(Z010)、日本结缕草(Z122)、马尾草(Z095)和结缕草(Z115)单独聚为一类部分次级类群的聚类结果表明，大部分日本大枣和中华大枣聚为一类，马尾藻和小叶大枣聚为一类，日本大枣、中华大枣和小叶大枣的部分种质聚为一类。同时，对结缕草种质资源的归属问题进行了初步探讨，认为本研究结果可为结缕草种质资源的进一步研究和应用提供分子水平上的科学依据。
The genetic diversity and interspecific relationship of 96 germplasms of Zoysia Willd.belonging to 5 species and 1 mutation were analyzed using SSR(Simple Sequence Repeat) markers.The results showed that:(1)Total 282 bands were detected by 29 pairs of SSR primers,and 272 of 282 were polymorphic with PPB(Percentage of Polymorphic Bands) at 96.45%,the Nei's gene diversity index(H) was 0.2203,and the Shannon diversity index(I) was 0.3504,indicating a high level of genetic diversity in zoysiagrass.(2) The GS(genetic similarity) among 96 Zoysia germplasms ranged from 0.5922 to 0.9362 with the average of 0.7811.GS between Z.japonica and Z.sinica and between Z.matrella and Z.tenuifolia were relatively high,both Z.machrostachya(Z010) and Z.sinica.var.nipponica(Z122) had a relatively lower GS than other species.The range of GS within species of Z.japonica,Z.sinica,and Z.matrella were 0.5922~0.9362,0.6879~0.9078,and 0.6738~0.8582,respectively.(3) Genetic distance and relationship among 96 Zoysia germplasms were showed clearly in the cluster dendrogram.The 96 Zoysia germplasms were classified into 6 major groups by cluster analysis.Group I included Z.Japonica,Z.sinica,and some accessions of Z.matrella,accounted for 90.6% of all germplasms.Some accessions of Z.matrella and Z.tenuifolia form one group.Z.machrostachya(Z010),Z.sinic var.nipponica(Z122),Z. matrella(Z095),and Z.sinica(Z115) form one group by itself,respectively.The cluster results of some secondary groups showed that most accessions of Z.japonica and Z.Sinica were clustered to one group,whereas,Z.matrella and Z.tenuifolia were one group,and some accessions of Z.japonica,Z.Sinica and Z.matrella were one group.In this research,the diversity and relationship among 96 germplasms were displayed;meanwhile,a preliminary discussion was made on the ownership of part accessions.We consider the findings could provide a scientific basis at the molecular level for future study and application of zoysiagrass germplasms.