Comparative genomics of Shiga toxin encoding bacteriophages.
Comparative genomics of Shiga toxin encoding bacteriophages.
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DOI：
10.1186/1471-2164-13-311
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发表时间：
2012-07-16
期刊：
影响因子：
4.4
通讯作者：
Allison HE
中科院分区：
文献类型：
--
作者：
Smith DL;Rooks DJ;Fogg PC;Darby AC;Thomson NR;McCarthy AJ;Allison HE
Stx噬菌体负责驱动Stx毒素基因（stx）在其细菌宿主范围内的传播。携带Stx毒素的溶原可引起严重的、危及生命的疾病，而Stx毒素是一种不可或缺的毒力因子。Stx-噬菌体vB_EcoP-24 B，通常被称为vB_EcoP-24 B，能够以高频率多重感染单个细菌宿主细胞，其中二次感染增加随后噬菌体感染可能发生的速率。这在生物学上是不寻常的，因此确定与其他类梭状芽孢杆菌Stx β相比，β 24 B的基因组含量和背景对于理解控制这种现象的因素并确定它们是否发生在其他Stx β中很重要。对Stx 2编码噬菌体的基因组测序并注释。基因组组织和一般特征类似于其他测序的Stx噬菌体诱导肠出血性大肠杆菌（EHEC），但STX 24 B具有显着的异质性区域，与噬菌体生物学和行为的影响。使用Circos基因组比较工具和基于PCR的多基因座比较系统，将STX 24 B基因组与其他测序的Stx噬菌体和原型类噬菌体λ进行比较。这些数据支持以下假设，即Stx噬菌体是嵌合的，并且宿主、噬菌体和它们在同一感染的细菌细胞内的残余物之间的重组事件将继续驱动Stx噬菌体变体的进化和随后的产志贺毒素潜力的传播。
Stx bacteriophages are responsible for driving the dissemination of Stx toxin genes (stx) across their bacterial host range. Lysogens carrying Stx phages can cause severe, life-threatening disease and Stx toxin is an integral virulence factor. The Stx-bacteriophage vB_EcoP-24B, commonly referred to as Ф24B, is capable of multiply infecting a single bacterial host cell at a high frequency, with secondary infection increasing the rate at which subsequent bacteriophage infections can occur. This is biologically unusual, therefore determining the genomic content and context of Ф24B compared to other lambdoid Stx phages is important to understanding the factors controlling this phenomenon and determining whether they occur in other Stx phages. The genome of the Stx2 encoding phage, Ф24B was sequenced and annotated. The genomic organisation and general features are similar to other sequenced Stx bacteriophages induced from Enterohaemorrhagic Escherichia coli (EHEC), however Ф24B possesses significant regions of heterogeneity, with implications for phage biology and behaviour. The Ф24B genome was compared to other sequenced Stx phages and the archetypal lambdoid phage, lambda, using the Circos genome comparison tool and a PCR-based multi-loci comparison system. The data support the hypothesis that Stx phages are mosaic, and recombination events between the host, phages and their remnants within the same infected bacterial cell will continue to drive the evolution of Stx phage variants and the subsequent dissemination of shigatoxigenic potential.
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影响因子：
3.2
作者：
Davis, BM;Moyer, KE;Waldor, MK
通讯作者：
Waldor, MK
影响因子：
5.4
作者：
Berngruber, Thomas W.;Weissing, Franz J.;Gandon, Sylvain
通讯作者：
Gandon, Sylvain
影响因子：
14.9
作者：
Grant JR;Stothard P
通讯作者：
Stothard P
影响因子：
14.9
作者：
Fogg PC;Rigden DJ;Saunders JR;McCarthy AJ;Allison HE
通讯作者：
Allison HE
影响因子：
4.5
作者：
Hatfull GF;Pedulla ML;Jacobs-Sera D;Cichon PM;Foley A;Ford ME;Gonda RM;Houtz JM;Hryckowian AJ;Kelchner VA;Namburi S;Pajcini KV;Popovich MG;Schleicher DT;Simanek BZ;Smith AL;Zdanowicz GM;Kumar V;Peebles CL;Jacobs WR Jr;Lawrence JG;Hendrix RW
通讯作者：
Hendrix RW