Localization of the human myelin basic protein gene (MBP) to region 18q22----qter by in situ hybridization.
Localization of the human myelin basic protein gene (MBP) to region 18q22----qter by in situ hybridization.
复制标题
通过原位杂交将人髓磷脂碱性蛋白基因 (MBP) 定位于 18q22----qter 区域。
DOI：
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发表时间：
1985
期刊：
影响因子：
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通讯作者：
M. Simon
中科院分区：
文献类型：
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作者：
D. Saxe;N. Takahashi;L. Hood;M. Simon
来自人基因组DNA克隆部分的限制性内切酶片段对应于髓鞘碱性蛋白基因，已被用于通过原位杂交定位该基因到人中期染色体的位置。10%的放射性标记位点位于18号染色体上。18号染色体上84%的谷粒位于18q22-qter区域。这表示在该位置的标记比沿沿着所有其他染色体发现的背景颗粒分布增加了10倍以上。
A restriction endonuclease fragment derived from a cloned portion of human genomic DNA corresponding to the myelin basic protein gene has been used to map the position of this gene by in situ hybridization to human metaphase chromosomes. Ten percent of the radioactively labeled sites observed were on chromosome 18. Eighty-four percent of the grains on chromosome 18 were located within the region corresponding to 18q22----qter. This represents a greater than 10-fold increase in labeling at this position over the background grain distribution found along all of the other chromosomes.