Candida albicans RIM101 pH response pathway is required for host-pathogen interactions
Candida albicans RIM101 pH response pathway is required for host-pathogen interactions
复制标题
DOI：
10.1128/iai.68.10.5953-5959.2000
复制
发表时间：
2000-10-01
影响因子：
3.1
通讯作者：
Ibrahim, AS
中科院分区：
文献类型：
--
作者：
Davis, D;Edwards, JE;Ibrahim, AS
白色念珠菌对不同环境的反应能力是其成功作为病原体的关键。白念珠菌对体外环境pH值变化的反应。在这项研究中，我们发现RIM 101途径是体内致病所必需的。首先，我们使用血源性播散性系统性念珠菌病小鼠模型显示rim 101（-/）rim 101（-）和rim 8（-）/rim 8（-）突变体具有显著的毒力降低。第三，rim 101（-）/rim 101（-）和rim 8（-）/rim 8（-）突变体在原位损伤内皮细胞的能力上显示出缺陷。最后，我们证明了RIM 101的激活等位基因RIM 101 -405是体内rim 8（-）突变的抑制因子，因为它挽救了rim 8（-）/rim 8（-）突变体的毒力、组织学和内皮损伤缺陷。这些结果表明，Rim 101途径是体内白念珠菌毒力所必需的，并且Rim 8 p在发病机制中的功能是激活Rim 101 p。
The ability of Candida albicans to respond to diverse environments is critical for its success as a pathogen, The RIM101 pathway controls gene expression and the yeast-to-hyphal transition in C. albicans in response to changes in environmental pH in vitro. In this study, we found that the RIM101 pathway is necessary in vivo for pathogenesis, First, we show that rim101(-/)rim101(-) and rim8(-)/rim8(-) mutants have a significant reduction in virulence using the mouse model of hematogenously disseminated systemic candidiasis, Second, these mutants show a marked reduction in kidney pathology. Third, the rim101(-)/rim101(-) and rim8(-)/rim8(-) mutants show defects in the ability to damage endothelial cells in situ, Finally, we show that an activated allele of RIM101, RIM101-405, is a suppressor of the rim8(-) mutation in vivo as it rescues the virulence, histological, and endothelial damage defects of the rim8(-)/rim8(-) mutant. These results demonstrate that the RIM101 pathway is required for C, albicans virulence in vivo and that the function of Rim8p in pathogenesis is to activate Rim101p.