Green synthesis of stable platinum nanoclusters with enhanced peroxidase-like activity for sensitive detection of glucose and glutathione
Green synthesis of stable platinum nanoclusters with enhanced peroxidase-like activity for sensitive detection of glucose and glutathione
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DOI：
10.1016/j.microc.2021.106202
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发表时间：
2021-04-09
影响因子：
4.8
通讯作者：
Wang, Longgang
中科院分区：
文献类型：
--
作者：
Fan, Liyuan;Ji, Xianbing;Wang, Longgang
灵敏且便捷地检测葡萄糖和谷胱甘肽（GSH）的浓度对我们的健康至关重要。在本研究中，通过一种简便的合成策略制备了百合多糖（LP）稳定的铂纳米团簇（Ptn - LP NCs），其中LP用作还原剂和稳定剂。分别利用紫外 - 可见光谱、X射线光电子能谱（XPS）、傅里叶变换红外光谱（FTIR）和透射电子显微镜（TEM）证实了在Ptn - LP NCs内部成功制备出了1 - 2纳米的铂纳米团簇（Pt NCs）。此外，在过氧化氢（H₂O₂）存在的情况下，Ptn - LP NCs可加速无色的3,3',5,5'-四甲基联苯胺（TMB）催化氧化为蓝色的氧化态TMB（oxTMB），这表明其具有内在的类过氧化物酶活性。TMB和H₂O₂的Vm值分别为50.3×10⁻⁸ M s⁻¹和10.2×10⁻⁸ M s⁻¹，高于其他模拟酶。Ptn - LP NCs的类过氧化物酶特性被用于灵敏地检测葡萄糖和谷胱甘肽（GSH）的浓度。葡萄糖和GSH的检测限分别低至1.89 μM和0.37 μM，葡萄糖和GSH的线性范围分别为2.5 - 1000 μM和4 - 140 μM。该比色法已成功应用于检测人血清中的GSH浓度。这种方法在未来生物相关检测中具有巨大的潜在应用价值。
The sensitive and facile detection of the concentration of glucose and glutathione (GSH) is important for our health. In this work, lily polysaccharide (LP) stabilized platinum nanoclusters (Ptn-LP NCs) were prepared by a facile synthetic strategy where LP was used as a reducing agent and stabilizer. The successful preparation of 1-2 nm platinum nanoclusters (Pt NCs) inside of Ptn-LP NCs was confirmed using UV-Vis spectra, X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR) and Transmission electron microscopy (TEM), respectively. Furthermore, Ptn-LP NCs accelerated the catalytic oxidation of colorless 3,3 ',5,5 ' tetramethylbenzidine (TMB) to blue oxidized TMB (oxTMB) in the presence of hydrogen peroxide (H2O2), indicating their intrinsic peroxidase-like activity. The Vm values of TMB and H2O2 were 50.3 x 10-8 Ms- 1 and 10.2 x 10-8 Ms- 1, respectively, which were higher than those of other mimetic enzymes. The peroxidase-like property of Ptn-LP NCs was used to sensitively detect the concentration of glucose and glutathione (GSH). The detection limits of glucose and GSH were as low as 1.89 and 0.37 mu M, the linear ranges of glucose and GSH were 2.5-1000 and 4-140 mu M, respectively. The colorimetric method was successfully applied to detect GSH concentration in human serum. This method has great potential application in bio-related detection in the future.