Temporal and spatial control of cyclin B1 destruction in metaphase
Temporal and spatial control of cyclin B1 destruction in metaphase
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DOI：
10.1038/10049
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发表时间：
1999-06-01
影响因子：
21.3
通讯作者：
Pines, J
中科院分区：
文献类型：
--
作者：
Clute, P;Pines, J
关键调节蛋白的蛋白水解被认为是通过有丝分裂控制进程。在这里，我们分析了细胞周期蛋白B1降解的真实的时间和Sind，它开始尽快最后一个染色体在中期板上对齐，就在纺锤体组装检查点被灭活。在这一点上，细胞周期蛋白B1染色从纺锤体极和染色体上消失。如果重新施加纺锤体检查点，则细胞周期蛋白B1的破坏随后可以在整个中期失活，这与细胞周期蛋白B1在纺锤体极和染色体上的再现相关。这些结果提供了一个时间和空间之间的联系纺锤体组装检查点和泛素介导的蛋白质水解。
The proteolysis of key regulatory proteins is thought to control progress through mitosis. Here we analyse cyclin B1 degradation in real time and Sind that it begins as soon as the last chromosome aligns on the metaphase plate, just after the spindle-assembly checkpoint is inactivated. At this point, cyclin B1 staining disappears from the spindle poles and from the chromosomes. Cyclin B1 destruction can subsequently be inactivated throughout metaphase if the spindle checkpoint is reimposed, and this correlates with the reappearance of cyclin B1 on the spindle poles and the chromosomes. These results provide a temporal and spatial link between the spindle-assembly checkpoint and ubiquitin-mediated proteolysis.